Different proteins could annotate a single mass peak of a mass spectrum

Functional annotation of mass spectrum of bacterial cells obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) generates the biological meaning that underpins the use of mass spectrometry for microbial identification. However, different proteins could have the same mass/charge ratio and thus able to annotate the same mass peak in a mass spectrum. Such … More Different proteins could annotate a single mass peak of a mass spectrum

Utility of rescue experiments in biology

Loss of function assays are common in biology for understanding the function associated with a protein or gene. Typically, targeted mutagenesis is used for creating a specific gene or protein mutant, that due to loss of critical sequence information, result in a loss of function. Thus, the function lost can be associated with the gene … More Utility of rescue experiments in biology

Phylogenetic trees track the evolutionary relatedness of genes and proteins

The tree of life is currently defined by a phylogenetic analysis of the 16S rRNA gene, which harbours deep evolutionary information on the development of various species on Earth. Specifically, it is highly conserved across species, and yet possesses sufficient variability for individual species to be identified by differential sequence at the gene level. Thus, … More Phylogenetic trees track the evolutionary relatedness of genes and proteins

Computer modelling for fitting possible structures to diffraction data

Modern structural biology is a combination of diffraction experiments using X-rays or electron, and computer simulation for fitting possible structures to the diffraction data.   Specifically, after protein crystallization and determination of structure through X-ray diffraction or cryo-electron microscopy, the resulting data remains difficult to understand. Thus, molecular simulation tools such as molecular dynamics are … More Computer modelling for fitting possible structures to diffraction data

Understanding the evolutionary dynamics of phosphorylation with fungal species as model system

Phosphorylation plays important roles in many aspects of cell biology and intra- and intercellular signaling; for example, serving as markers for epigenetic regulation of gene transcription, and more commonly, as a repository of energy for enzymatic and protein function. But, what determines the selection of specific protein sequence motif as sites for inserting a phosphorus … More Understanding the evolutionary dynamics of phosphorylation with fungal species as model system