Gut microbiome changes in sync with food intake pattern

Gut microbiome is the consortium of microorganisms that inhabit the mucous membrane lining the large and small intestines, and is responsible for many body functions such as digestion of food and regulation of overall intestinal health. A recent study reported in the New York Times (Link) reveals that the gut microbiome of a population in … More Gut microbiome changes in sync with food intake pattern

Difficulty of separating and fractionating complex biological medium

Understanding the composition of a growth medium is essential to full knowledge of the signalling, metabolite exchanges, and byproducts generated during growth of cells in the medium. But, the endeavour is usually fraught with difficulties of separating or fractionating different components present in the medium by a variety of instrumented techniques such as high performance … More Difficulty of separating and fractionating complex biological medium

Single cell transcriptomics for revealing the compendium of different cell fates and cellular differentiation

Genomics is commonly thought to be the method for understanding the functional repertoire of cells, which, by extension, would reveal the different cell types, fates, lineages and cellular differentiation available to a consortium of cells within a locale such as the human body.   However, many of the genes encoded within the genome of a … More Single cell transcriptomics for revealing the compendium of different cell fates and cellular differentiation

Bacterial immune system utilized for genome editing

Feature article in Nature, Vol. 541, Issue 7637, pp. 280-282, “CRISPR’s Mysteries”   Summary of article: Used by bacterial and archaeal species for immune surveillance and defence of the cell against viruses, the single cell organism’s immune system, clustered regularly interspaced short palindromic repeats (CRISPR) system has increasingly find use in alternate scenarios, the most … More Bacterial immune system utilized for genome editing

Understanding Earth’s collection of viruses

Article in Nature, Vol. 536, Issue 7617, pp. 425-430, “Uncovering Earth’s virome”   Summary of article: A vector based metagenomics approach combined with bioinformatics analysis was used to uncover the subset of viruses present on human body surfaces and freshwater habitats. The study’s experiment design does not fit the grandiose nature of the paper’s title. … More Understanding Earth’s collection of viruses

High precision sequencing of 16S rRNA gene, important for phylogenetic purposes

Article in PeerJ, “A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq”   Summary of article: A tag sequencing method utilizing the Ilumina MiSeq platform was shown to be useful in sequencing, in high precision, near full length 16S rRNA gene, which is important for phylogenetic purposes, particularly … More High precision sequencing of 16S rRNA gene, important for phylogenetic purposes

Collection of microbes in the gastrointestinal tract changes with the diet we eat

Microbes are all around us; in the air we breathe and surfaces we touch. But, we don’t fall sick from exposure to microbes. Why? Because our bodies possess an immune system capable of defending against harmful microbes when we are healthy. More importantly, many of the microbes we are exposed to are harmless, and maybe … More Collection of microbes in the gastrointestinal tract changes with the diet we eat

Ethanol pretreatment and bile acid germinants help recover significant number of spore forming gut bacteria previously unculturable

Microbes are the dominant forms of life on Earth both in numbers (6 x 1030) and species. By mediating diverse biogeochemical processes ranging from photosynthesis to leaching of metals from minerals and methane biogenesis, they are essential to the proper functioning of the planet. Nevertheless, most of the microorganisms could not be cultivated in the … More Ethanol pretreatment and bile acid germinants help recover significant number of spore forming gut bacteria previously unculturable

Post antibiotic treatment expansion of pathogenic gut microbes induced by availability of oxidised sugars

Antibiotic resistance is frequently publicized in the popular media such as television advertisement and newspapers and has helped inform the general public about the perils of not completing a course of antibiotic treatment. Briefly, antibiotic resistance arises when a subpopulation of microorganisms developed resistance to antibiotic treatment either through reducing their metabolic rate (e.g., persistence … More Post antibiotic treatment expansion of pathogenic gut microbes induced by availability of oxidised sugars