Antibiotic resistance emerged prior to the advent of antibiotics medication

Antibiotics are natural compounds isolated from microorganisms useful for fending off other microbes in natural settings. Thus, resistance pathways, mechanisms, and genes emerged in microorganisms prior to the advent of antibiotics medication. What is commonly known as emergence of antibiotics resistance in the contemporary era refers to the horizontal transfer of resistance genes between microbes … More Antibiotic resistance emerged prior to the advent of antibiotics medication

Bacterial species in the Vibrio genus have two chromosomes instead of one

Genome sequencing studies have revealed that bacterial species in the Vibrio genus have two chromosomes instead of one. Typically, bacterial species genome is defined by one single circular chromosome, not two. The observations opened up many avenues for further research such as understanding the reasons why marine and aquatic Vibrio species are endowed with two … More Bacterial species in the Vibrio genus have two chromosomes instead of one

Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Bacillus pumilus is a Gram-positive endospore forming bacterium capable of withstanding high intensity ultraviolet radiation. However, genome sequencing studies have revealed that the bacterium lack important genes in DNA repair compared to other bacterial species. Thus, it raises an important question concerning the mechanisms that underpins the enhanced resistance of the bacterium to high intensity … More Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Glucose supplementation is commonly used to provide more carbon source to a medium to help facilitate the attainment of a greater cell density. However, from my personal experience in microbial cell culture, excessive glucose supplementation would reduce biomass formation, possibly due to the induction of overflow metabolism through the central carbon pathway that lead to … More Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Conserved mass peaks of bacteria at the genus level

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been validated to be useful in identifying many microbial species to the species level. However, the approach relies on the existence of different mass peaks between different microbial species. Such differences manifest as differences in mass peak distribution in mass spectra of microbes that could be … More Conserved mass peaks of bacteria at the genus level

Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Swarming motility is the collective coordinated movement of bacterial cells across a solid surface for colonizing new habitats such as during times of nutritional stress. Propelled by multiple flagella attached to the cell and aided by secreted surfactants and lubricants, swarming motility typically forms complex motility patterns during culture of the bacteria on solid agar … More Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Theoretical ribosomal protein mass fingerprint database

Ribosomal proteins constitute the large and small subunit of the ribosome. Given the highly conserved nature of ribosomes, ribosomal proteins are expected to be similarly conserved. However, theoretical calculations of the molecular mass of ribosomal proteins of different species revealed the existence of distinct molecular mass fingerprint of ribosomal proteins from individual species. Such ribosomal … More Theoretical ribosomal protein mass fingerprint database

Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies

Bacillus subtilis is a common Gram-positive soil bacterium with important applications in biotechnology. Specifically, it is frequently used as the production host for a variety of value-added products or pharmaceuticals. Possessing a variety of differentiation pathways for coping with nutrient starvation, the bacterium is also a model organism for studying how bacteria cope with low … More Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies

Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

Biotechnology production typically relies on bioreactor fermentation for producing needed compounds, whether for nutritional or pharmaceutical uses. However, stirring and aeration necessary for bioreactor fermentation generates foam that pose a risk to contamination by unwanted microbes. Thus, antifoam such as polyethylene glycol (PEG) are commonly used to reduce foam formation under aggressive aeration or stirring. … More Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

Microbes in deionized and tap water

Large variety of microorganisms were recovered from deionized and tap water after cultivation on R2A agar and a formulated colourless agar. Specifically, more types and numbers of microorganisms were recovered from deionized water compared to tap water due possibly to the removal of the residual disinfectant monochloramine from tap water by ion exchange resins of … More Microbes in deionized and tap water