Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Glucose supplementation is commonly used to provide more carbon source to a medium to help facilitate the attainment of a greater cell density. However, from my personal experience in microbial cell culture, excessive glucose supplementation would reduce biomass formation, possibly due to the induction of overflow metabolism through the central carbon pathway that lead to … More Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Swarming motility is the collective coordinated movement of bacterial cells across a solid surface for colonizing new habitats such as during times of nutritional stress. Propelled by multiple flagella attached to the cell and aided by secreted surfactants and lubricants, swarming motility typically forms complex motility patterns during culture of the bacteria on solid agar … More Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Informatic challenge in MALDI-TOF imaging mass spectrometry

Imaging mass spectrometry is a relatively new concept in mass spectrometry application that seeks to profile the ensemble of biomolecules in a tissue or sample. Useful for detecting and analysing biomolecules present in a sample in a large area format, imaging mass spectrometry generates huge datasets that must be annotated to obtain rich biological information … More Informatic challenge in MALDI-TOF imaging mass spectrometry

Theoretical ribosomal protein mass fingerprint database

Ribosomal proteins constitute the large and small subunit of the ribosome. Given the highly conserved nature of ribosomes, ribosomal proteins are expected to be similarly conserved. However, theoretical calculations of the molecular mass of ribosomal proteins of different species revealed the existence of distinct molecular mass fingerprint of ribosomal proteins from individual species. Such ribosomal … More Theoretical ribosomal protein mass fingerprint database

Inferior solutions from single pathway-based drug discovery

Biomedical research typically focuses on a few pathways thought to be important to disease physiology; however, multiple pathways (both signalling and metabolic) are likely to be important to the clinical manifestations of complex diseases such as metabolic syndrome or cancer. Hence, what are the repercussions from biomedical research focus on single pathway for understanding disease … More Inferior solutions from single pathway-based drug discovery

General structure of the ribosome may be conserved, but small alterations in structural features likely exist between ribosomes of different species

Ribosome are highly conserved in structure and function given their importance to protein synthesis. Comprising a small and large subunit, ribosomes are built up of ribosomal proteins and RNA that provide both structure and function to the macromolecular complex.   Ribosomal proteins, in particular, differ in mass between different species and thus constitute a unique … More General structure of the ribosome may be conserved, but small alterations in structural features likely exist between ribosomes of different species

Long flight tube in time-of-flight mass spectrometer enables better separation of different analyte ions

Molecular ions literally fly through the flight tube of the time-of-flight mass spectrometer where at the end of the travel, they are detected as an electrical charge on the detector. The duration of flight was measured and is a key parameter for calculation of the mass/charge ratio of the molecular ion based on a calibration … More Long flight tube in time-of-flight mass spectrometer enables better separation of different analyte ions

Is there a theoretical mass limit to MALDI-TOF mass spectrometry?

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) uses a laser to ionize molecules from a matrix coated sample surface, accelerate them along a vacuum tube through the application of an electric field, and obtained a mass/charge ratio of the molecular ion by timing the flight of the ion. Given that time-of-flight of the molecular … More Is there a theoretical mass limit to MALDI-TOF mass spectrometry?

Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) generates microorganism-specific mass spectrum which has been shown to be useful in microbial identification via the mass spectrum fingerprinting approach. Specifically, the mass spectrum of an unknown microorganism is compared against those catalogued in a reference database of known microbes to aid in positive identification of microbe. … More Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

Inability to constrain metabolic models with growth data in the absence of compositional changes in medium

The goal of metabolic modelling is to determine the metabolic flux through specific pathways in the cell. Doing so requires data on cell growth (whether microbes or human cells) as well as compositional data of the growth medium given that cellular metabolic activities would consume nutrients as well as generate byproducts that accumulate in the … More Inability to constrain metabolic models with growth data in the absence of compositional changes in medium