Bacterial species in the Vibrio genus have two chromosomes instead of one

Genome sequencing studies have revealed that bacterial species in the Vibrio genus have two chromosomes instead of one. Typically, bacterial species genome is defined by one single circular chromosome, not two. The observations opened up many avenues for further research such as understanding the reasons why marine and aquatic Vibrio species are endowed with two … More Bacterial species in the Vibrio genus have two chromosomes instead of one

Effect of wash buffers on zeta potential measurement of bacterial cells

Nonspecific adsorption of ions and metabolites from the growth medium on the bacterial cell surface is inevitable given the presence of various functional groups on the cell envelope. Thus, various wash buffers such as phosphate buffered saline and 0.1M sodium nitrate have been used in repeated steps of centrifugation and washing for removing the nonspecifically … More Effect of wash buffers on zeta potential measurement of bacterial cells

Colourless agar for enhancing colour contrast between microbial colonies and agar

Small microbial colonies on agar could be hard to discern if the background colour of the agar is close to that of the colonies. This pose a significant problem for both manual and automated identification of microbial colonies. By separately sterilizing glucose and ammonium compounds, a recent study developed a colourless agar that enhanced the … More Colourless agar for enhancing colour contrast between microbial colonies and agar

Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) generates microorganism-specific mass spectrum which has been shown to be useful in microbial identification via the mass spectrum fingerprinting approach. Specifically, the mass spectrum of an unknown microorganism is compared against those catalogued in a reference database of known microbes to aid in positive identification of microbe. … More Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

How to confirm that a microbe could grow in minimal salts medium?

Growth in a minimal salts medium signifies that a microbe has a competitive advantage over other microbial species for nutrients and, more importantly, environmental habitats. But, how should one confirm that a microbe could grow in minimal salts medium? Specifically, should the inoculum contain any trace of a rich medium? The answer to the latter … More How to confirm that a microbe could grow in minimal salts medium?

Health concerns of using grey water to flush toilets

Water conservation is the main theme of discussion amongst future city planners and public utilities experts, and grey water is increasingly being put forward as a possible source of water for flushing toilets, as cities bid to reduce their overall water consumption. But, what is grey water and is it safe as a water source … More Health concerns of using grey water to flush toilets