Tag: Escherichia coli

Recap of my research journey (2004 to present)

April 19, 2024

My research journey started in May 2004 with a UROP (Undergraduate Research Opportunities Programme) project on biosorption of copper using local marine seaweed in Prof. Chen Jiaping’s lab at National University of Singapore. Currently, I am working on developing quantitative polymerase chain reaction (qPCR) assay and device for detecting Helicobacter pylori in stomach gastric samples. … More Recap of my research journey (2004 to present)

Sanger sequencing is a robust technique with few sequencing errors

June 13, 2023

Gene sequencing is a common technique used in a variety of biological research workflows. Currently, the gold standard method remains Sanger sequencing due to its reliability and low error rate. This work reports a small retrospective analysis of past Sanger sequencing data to assess the extent of low error rate that is inherent in the … More Sanger sequencing is a robust technique with few sequencing errors

Recoded synthetic Escherichia coli genome

November 18, 2020

Scientists have been pursuing the synthesis of genome of microorganisms for improving our capabilities in many areas of biological research. One area that would benefit is our ability to synthesize long DNA molecules. Another area that would see advancement is our ability to introduce long DNA molecules into living microorganisms. Additionally, we would also be … More Recoded synthetic Escherichia coli genome

Model organisms serve as representative to dissect complex biology of different species

September 11, 2020

Biology is characterized by its immense complexity. From animals to plants and bacteria, multitude of different species could co-exist in the same habitat accounting for the rich biodiversity of the world. Thus, how should one begin to unentangle the complexity inherent in biological systems? The answer comes from the use of model organisms to delve … More Model organisms serve as representative to dissect complex biology of different species

Zeta potential of Escherichia coli DH5α cells grown in different growth media

January 15, 2020

Bacterial cells adapt to growth in different growth medium. Thus, could the cell surface structures and, by extension, surface charge characteristics of bacterial cells change as a result of growth in different growth medium. In addition, different growth medium comprises different sets of ions and metabolites for aiding growth of cells, could these ions and … More Zeta potential of Escherichia coli DH5α cells grown in different growth media

Recent research shows that prokaryotic cells could stably maintain two large synthetic circular chromosomes

September 7, 2019

Prokaryotic cells such as those of bacteria are characterized by the lack of internal compartments (or organelles), absence of a nucleus, and the presence of one circular chromosome. Evolution has endowed prokaryotic cells with the ability to stably maintain and replicate this circular chromosome, which is the carrier of genetic information in the cell. Recent … More Recent research shows that prokaryotic cells could stably maintain two large synthetic circular chromosomes

Effect of wash buffers on zeta potential measurement of bacterial cells

March 19, 2018

Nonspecific adsorption of ions and metabolites from the growth medium on the bacterial cell surface is inevitable given the presence of various functional groups on the cell envelope. Thus, various wash buffers such as phosphate buffered saline and 0.1M sodium nitrate have been used in repeated steps of centrifugation and washing for removing the nonspecifically … More Effect of wash buffers on zeta potential measurement of bacterial cells

High cell density cultivation of Escherichia coli DH5α in shake flask

March 5, 2018

High cell density is desired in many biotechnology applications such as the generation of cells for characterization studies and performance tests in different scenarios. However, cell densities obtained during growth of bacteria in common growth media remained low; thereby, raising the issue of batch-to-batch variation in cell characteristics due to the need of using many … More High cell density cultivation of Escherichia coli DH5α in shake flask