Caution should be exercised in interpreting cryo-electron microscopy structures

Cryo-electron microscopy uses snap-freezing to fix the conformations of proteins prior to electron microscopy imaging. From the initial blurry images to high resolution electron density maps that could rival those obtained from X-ray crystallography, cryo-electron microscopy (cryo-EM) has come a long way in terms of innovations in technique and progressive incremental refinements. While the technique … More Caution should be exercised in interpreting cryo-electron microscopy structures

DNA writers for encoding cellular signals into the genome

Myriad cellular signals are generated through various regulatory activities for processes in metabolism and signal transduction. But the challenge has been in understanding these cellular signal transduction events that have largely remained hidden from our view. Using DNA writing technologies, researchers have been able to encode some of these previously hidden intracellular messages and signals … More DNA writers for encoding cellular signals into the genome

Enzyme likely evolved from a high temperature to a low temperature environment

Given that the last universal common ancestor (LUCA) cells likely existed in alkaline hydrothermal vents in the ocean, enzymes that exist by that time in life’s evolution should be ones adapted to high temperature environments. Thus, in progressing from a high to low temperature environment, enzymes likely took a path down the energy ladder in … More Enzyme likely evolved from a high temperature to a low temperature environment

LB Lennox medium is limiting in sugars not nitrogen

LB Lennox medium is commonly used for the cultivation of a variety of microorganisms such as bacteria. Comprising tryptone, yeast extract, and sodium chloride, its provides a rich medium for the cultivation of bacteria to relatively high cell density. However, while the attained cell density may be sufficient for cell biology and molecular biology studies, … More LB Lennox medium is limiting in sugars not nitrogen

Construction of single chromosome yeast cells

Yeast cells carry 16 linear chromosomes for encapsulating the genome of the species. Recent research has sought to rewrite the genome of yeast and one approach seeks to reduce the number of chromosomes required to encode all the genomic information. Specifically, by deleting the centromere and telomere of yeast chromosomes and fusing one chromosome with … More Construction of single chromosome yeast cells

Light-based patterning of bacterial cells through induction of biofilm formation processes

Microbes do not live alone, rather, they assemble into multi-species and multicellular assemblage known as biofilms on various surfaces. Specifically, microbial cells often assemble into biofilm matrix with differentiated functions and roles in the consortium resulting from complex intercellular communications and signalling that mediates a division of labour amongst cells of the biofilm. Architecturally complex … More Light-based patterning of bacterial cells through induction of biofilm formation processes

CRISPR interference could negate the need for construction of deletion mutants

Loss of function studies is commonly used to decipher the functions of genes. Multiple ways could be used to implement loss of function of genes such as inactivation of gene expression or construction of deletion mutants. Due to the problem of leaky expression, inactivation of gene expression might not completely abrogate the expression of a … More CRISPR interference could negate the need for construction of deletion mutants

MALDI-TOF mass spectrum of bacteria depends on their growth state

A mass spectrometer profiles for the set of molecules whose abundances exceed the minimum sensitivity of the instrument. Thus, highly abundant biomolecules would be more likely to be profiled and featured on the corresponding mass spectrum of the sample. In the case of profiling of biomolecules from microbial cells using the technique of matrix-assisted laser … More MALDI-TOF mass spectrum of bacteria depends on their growth state

Wash buffer of ionic strength 0.15M could unmask the real surface charge of Bacillus subtilis NRS-762 cells

Bacterial cells in solution would inevitably carry a layer of nonspecifically adsorbed ions and molecules on their cell surface resulting from contact with the solution. This layer of nonspecifically adsorbed ions and molecules would thus masks the real surface charge of the bacteria. To reveal the real surface charge of bacteria during microelectrophoretic measurement of … More Wash buffer of ionic strength 0.15M could unmask the real surface charge of Bacillus subtilis NRS-762 cells

Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Bacillus pumilus is a Gram-positive endospore forming bacterium capable of withstanding high intensity ultraviolet radiation. However, genome sequencing studies have revealed that the bacterium lack important genes in DNA repair compared to other bacterial species. Thus, it raises an important question concerning the mechanisms that underpins the enhanced resistance of the bacterium to high intensity … More Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032