Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Bacillus pumilus is a Gram-positive endospore forming bacterium capable of withstanding high intensity ultraviolet radiation. However, genome sequencing studies have revealed that the bacterium lack important genes in DNA repair compared to other bacterial species. Thus, it raises an important question concerning the mechanisms that underpins the enhanced resistance of the bacterium to high intensity … More Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Glucose supplementation is commonly used to provide more carbon source to a medium to help facilitate the attainment of a greater cell density. However, from my personal experience in microbial cell culture, excessive glucose supplementation would reduce biomass formation, possibly due to the induction of overflow metabolism through the central carbon pathway that lead to … More Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Conserved mass peaks of bacteria at the genus level

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been validated to be useful in identifying many microbial species to the species level. However, the approach relies on the existence of different mass peaks between different microbial species. Such differences manifest as differences in mass peak distribution in mass spectra of microbes that could be … More Conserved mass peaks of bacteria at the genus level

Informatic challenge in MALDI-TOF imaging mass spectrometry

Imaging mass spectrometry is a relatively new concept in mass spectrometry application that seeks to profile the ensemble of biomolecules in a tissue or sample. Useful for detecting and analysing biomolecules present in a sample in a large area format, imaging mass spectrometry generates huge datasets that must be annotated to obtain rich biological information … More Informatic challenge in MALDI-TOF imaging mass spectrometry

Theoretical ribosomal protein mass fingerprint database

Ribosomal proteins constitute the large and small subunit of the ribosome. Given the highly conserved nature of ribosomes, ribosomal proteins are expected to be similarly conserved. However, theoretical calculations of the molecular mass of ribosomal proteins of different species revealed the existence of distinct molecular mass fingerprint of ribosomal proteins from individual species. Such ribosomal … More Theoretical ribosomal protein mass fingerprint database

Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies

Bacillus subtilis is a common Gram-positive soil bacterium with important applications in biotechnology. Specifically, it is frequently used as the production host for a variety of value-added products or pharmaceuticals. Possessing a variety of differentiation pathways for coping with nutrient starvation, the bacterium is also a model organism for studying how bacteria cope with low … More Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies

Inferior solutions from single pathway-based drug discovery

Biomedical research typically focuses on a few pathways thought to be important to disease physiology; however, multiple pathways (both signalling and metabolic) are likely to be important to the clinical manifestations of complex diseases such as metabolic syndrome or cancer. Hence, what are the repercussions from biomedical research focus on single pathway for understanding disease … More Inferior solutions from single pathway-based drug discovery

Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

Biotechnology production typically relies on bioreactor fermentation for producing needed compounds, whether for nutritional or pharmaceutical uses. However, stirring and aeration necessary for bioreactor fermentation generates foam that pose a risk to contamination by unwanted microbes. Thus, antifoam such as polyethylene glycol (PEG) are commonly used to reduce foam formation under aggressive aeration or stirring. … More Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

High accuracy timing mechanisms in time-of-flight mass spectrometers enable high mass resolution detection

High accuracy timing in time-of-flight mass spectrometers facilitate the accurate detection of time of arrival of different molecular ions, which when compared to a calibration chart of protein standards with known molecular weight, enables the calculation of mass/charge ratio of high mass resolution. Specifically, accuracy of the timing mechanism at the millisecond timescale provides a … More High accuracy timing mechanisms in time-of-flight mass spectrometers enable high mass resolution detection

Effect of wash buffers on zeta potential measurement of bacterial cells

Nonspecific adsorption of ions and metabolites from the growth medium on the bacterial cell surface is inevitable given the presence of various functional groups on the cell envelope. Thus, various wash buffers such as phosphate buffered saline and 0.1M sodium nitrate have been used in repeated steps of centrifugation and washing for removing the nonspecifically … More Effect of wash buffers on zeta potential measurement of bacterial cells