Tag: genome editing

Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis

June 26, 2022

Transposase are “jumping” genes that excise themselves from one location in the genome to move to another. Thus, insertion of transposase genes into a new site in the genome may cause disruptions to gene function and mutability. Hence, by logical inference, bacterial species with high number of transposase and their helper protein genes are not … More Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis

Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis

October 24, 2021

Transposase are “jumping” genes that excise themselves from one location in the genome to move to another. Thus, insertion of transposase genes into a new site in the genome may cause disruptions to gene function and mutability. Hence, by logical inference, bacterial species with high number of transposase and their helper protein genes are not … More Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis

Transformation of bacterial cells with a plasmid encoding CRISPR-Cas9 machinery is still a necessary step for implementing the technique

May 24, 2021

Since its promulgation in 2012, CRISPR-Cas9 has emerged as the dominant technique for implementing genome editing in a variety of species and cell type. But, how is the technique implemented? What does the technique rely on? In essence, CRISPR-Cas9 genome editing requires a plasmid to encode both the guide RNA and Cas9 protein. This plasmid … More Transformation of bacterial cells with a plasmid encoding CRISPR-Cas9 machinery is still a necessary step for implementing the technique

Research in metabolic engineering is restricted to a few model organisms

January 6, 2021

Metabolic engineering is the scientific pursuit of engineering cells to produce desired chemicals and metabolites of use to society. These products could be drugs or nutraceuticals. Using the tools of modern genetic engineering and genome editing, endogenous metabolism of cells is rewired in metabolic engineering to redirect metabolic flux to desired pathways powering the production … More Research in metabolic engineering is restricted to a few model organisms

Focus in DNA synthesis research has increasingly shifted to genome construction

October 28, 2019

Advances in DNA writing technologies over the past decade has enabled the synthesis of DNA molecules of increasing length at declining cost. This has helped push forward the chemical synthesis of long stretches of DNA and has played an important role in facilitating the de novo synthesis of bacterial chromosome for Escherichia coli.   Focus … More Focus in DNA synthesis research has increasingly shifted to genome construction

Cas9 protein may not be able to access DNA encased in heterochromatin

August 2, 2019

CRISPR-Cas9 is the dominant gene editing technology in use. Utilizing the ability of guide RNA to specifically target any stretch of DNA, the system is able to guide the endonuclease Cas9 protein to the location indicated by the guide RNA to generate a double strand break that could serve as a locus for new DNA … More Cas9 protein may not be able to access DNA encased in heterochromatin

Generation of disease models through CRISPR-Cas genome editing technology

June 7, 2018

Lack of suitable disease models able to accurately recapitulate disease phenotypes is one of the major challenges in drug discovery and development given that disease models are needed for testing the relative efficacy of different candidate molecule at early stages of drug development. Thus, much effort have been devoted to the creation of suitable in … More Generation of disease models through CRISPR-Cas genome editing technology

Generation of disease models through CRISPR-Cas genome editing technology

April 9, 2018

Lack of suitable disease models able to accurately recapitulate disease phenotypes is one of the major challenges in drug discovery and development given that disease models are needed for testing the relative efficacy of different candidate molecule at early stages of drug development. Thus, much efforts have been devoted to the creation of suitable in … More Generation of disease models through CRISPR-Cas genome editing technology

Utility of CRISPR-Cas9 in genome editing lies in the error rate of DNA repair

December 16, 2017

Originally used in defending bacterial cells from attacks by viruses, clustered regularly interspersed short palindromic repeats (CRISPR) has been modified and utilized in editing single bases in DNA in a process known as genome editing. However, efficiency of CRISPR and CRISPR associated protein 9 (Cas9) in executing precise single nucleotide genome editing remains poor due … More Utility of CRISPR-Cas9 in genome editing lies in the error rate of DNA repair