Tag: ionic strength

Each recombinant protein needs its own refolding buffer

March 1, 2024

Recombinant proteins are typically synthesized as inclusion bodies in the genetically engineered microbial cells. In order to be functional, the recombinant protein needs to be refolded into its correct three-dimensional structure in vitro. To do this, a specialized reagent known as refolding buffer is employed. But, do all recombinant proteins share the same refolding buffer? … More Each recombinant protein needs its own refolding buffer

Explaining the manuscript: “Bacterial surface charge in layers”

June 18, 2022

During my master’s study, I undertook a project examining the effect of different wash buffers on bacterial surface charge measurement. Specifically, the hypothesis was that different wash buffers have different composition and ionic strength, and would thus exert different effects on removal of nonspecifically adsorbed ions and biomolecules on the cell surface. This would in … More Explaining the manuscript: “Bacterial surface charge in layers”

Nonspecific adsorption of ions and biomolecules mask the real surface charge of bacterial cells

February 15, 2020

Various proteins and biomolecules decorate the surface of bacterial cells. Whether tethered to the outer membrane of Gram-negative bacteria or adhering to the peptidoglycan layer of Gram-positive bacteria, these biomolecules endow the cell surface with an electrical charge and are responsible for various signalling and recognition processes mediated by cell surface receptors. However, in addition … More Nonspecific adsorption of ions and biomolecules mask the real surface charge of bacterial cells

Electroosmotic flow in zeta potential analysis

January 3, 2020

Microelectrophoresis light scattering is commonly used to measure the zeta potential of cells, which is a proxy parameter for the cell surface charge. Briefly, the technique entails the measurement of movement of cells within a fluid upon application of an electric field. Thus, depending on the amount of surface charge present on cells, different rates … More Electroosmotic flow in zeta potential analysis

Wash buffer of ionic strength 0.15M could unmask the real surface charge of Bacillus subtilis NRS-762 cells

July 26, 2018

Bacterial cells in solution would inevitably carry a layer of nonspecifically adsorbed ions and molecules on their cell surface resulting from contact with the solution. This layer of nonspecifically adsorbed ions and molecules would thus masks the real surface charge of the bacteria. To reveal the real surface charge of bacteria during microelectrophoretic measurement of … More Wash buffer of ionic strength 0.15M could unmask the real surface charge of Bacillus subtilis NRS-762 cells

Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

April 3, 2018

Biotechnology production typically relies on bioreactor fermentation for producing needed compounds, whether for nutritional or pharmaceutical uses. However, stirring and aeration necessary for bioreactor fermentation generates foam that pose a risk to contamination by unwanted microbes. Thus, antifoam such as polyethylene glycol (PEG) are commonly used to reduce foam formation under aggressive aeration or stirring. … More Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762

Effect of wash buffers on zeta potential measurement of bacterial cells

March 19, 2018

Nonspecific adsorption of ions and metabolites from the growth medium on the bacterial cell surface is inevitable given the presence of various functional groups on the cell envelope. Thus, various wash buffers such as phosphate buffered saline and 0.1M sodium nitrate have been used in repeated steps of centrifugation and washing for removing the nonspecifically … More Effect of wash buffers on zeta potential measurement of bacterial cells

Conventional drinking water treatment

September 1, 2017

All over the world, drinking water treatment comprises a specific set of steps designed to progressively remove various types of contaminants. Specifically, drinking water treatment comprises sedimentation, coagulation, filtration, sedimentation and disinfection.   When raw water first arrives at a drinking water treatment plant, it typically carries a heavy load of particles and is muddy … More Conventional drinking water treatment

Conventional drinking water treatment

September 1, 2017

All over the world, drinking water treatment comprises a specific set of steps designed to progressively remove various types of contaminants. Specifically, drinking water treatment comprises sedimentation, coagulation, filtration, sedimentation and disinfection. When raw water first arrives at a drinking water treatment plant, it typically carries a heavy load of particles and is muddy in … More Conventional drinking water treatment