Publications

Master’s thesis

Wenfa Ng, “Methodology development for environmental microbiology: Medium formulation and surface charge analysis”, Link to thesis

Publications

Book

  1. Wenfa Ng, “Software Tools and Methods for Metabolic Engineering: Protocols for Metabolic Engineering Research”
  2. Wenfa Ng, “Short Essays on Application Potential and Challenges of RNA-seq”
  3. Wenfa Ng, “Exploring the Biotechnological Potential of C2 Alternative Substrates in Biocatalysis and Metabolic Engineering”
  4. Wenfa Ng, “Illustrating How Analytical Approaches Can Be Used to Dissect Problems in Science and Engineering”

Book chapter

  1. Wenfa Ng, “Mouse xenograft models for elucidating resistance mechanisms to cancer therapeutics”, Horizons in Cancer Research, Vol. 82, Chapter 3
  2. Wenfa Ng, “Possible Odour-Mediated Attraction of Flies to Bacillus subtilis NRS-762 Stationary Phase Culture”, Advances in Medicine and Biology, Vol. 196, Chapter 5
  3. Wenfa Ng, “Categorization of prophage genes in Bacillus subtilis 168 and assessing their relative importance through RNA-seq gene expression analysis”, Advances in Biology, Vol. 2

Journal articles

  1. Wenfa Ng, “Production of Coenzyme Q10 in Recombinant Schizosaccharomyces

pombe Through Metabolic Engineering Approaches”, International Journal of Food and Nutrition, Vol. 1, Issue 2, pp. 1-12

2. Wenfa Ng, “Analysis on the sample types compatible with different types of SARSCoV-2 diagnostic test”, Asian Journal of Biomedical and Pharmaceutical Sciences, Vol. 11, Issue 81, pp. 1-3, Link to article

3. Wenfa Ng, “Microbes in deionized and tap water: Implications for maintenance of laboratory water production system”, Journal of Probiotics and Health, Accepted for publication

4. Wenfa Ng, “Number of mismatches and length of longest match correlate with alignment score in swalign built-in function in MATLAB”, Journal of Bioinformatics and Systems Biology, Vol. 4, Issue 4, pp. 104-107, Link to article

5. Wenfa Ng, “Existence of theoretical ribosomal protein mass fingerprints in bacteria, archaea and eukaryotes”, Ecology and Conservation Science: Open Access, Vol. 1, Issue 5, 555575, Link to article

6. Wenfa Ng, “Developing an ethanol utilization pathway based NADH regeneration system in Escherichia coli”, Journal of Biotechnology and Bioprocessing, Vol. 2, Issue 9, 059, Link to article

7. Wenfa Ng, “Colourless agar for enhancing colour contrast between microbial colonies and agar”, Journal of Food Science and Nutrition Research, Vol. 4, Issue 4, pp. 249-258, Link to article

8. Wenfa Ng, “Theoretical ribosomal protein mass fingerprint database”, Journal of Bioinformatics and Systems Biology, Vol. 4, Issue 4, pp. 140-143, Link to article

9. Wenfa Ng, “Evaluating the potential of applying machine learning tools to metabolic pathway optimization”, Journal of Biotechnology and Bioprocessing, Vol. 2, Issue 9, 060, Link to article

10. Wenfa Ng, “Annotation of ribosomal protein mass peaks in MALDI-TOF mass spectra of bacterial species and their phylogenetic significance”, Archives of Clinical and Biomedical Research, Vol. 5, Issue 6, December 2021, Link to article

11. Wenfa Ng, “Transmissible cancer in marine bivalves: Implications for mode of cancer transmission”, Journal of Surgery and Research, Vol. 4, Issue 4, pp. 588-590, Link to article

12. Wenfa Ng, “Employing statistical machine reading for inferring key concepts of a research field from a body of abstracts and blog posts”, Journal of Computer Science and Artificial Intelligence, Vol. 3, Issue 1, pp. 1-8, Link to article

13. Wenfa Ng, “Lack of mRNA transcripts of SARS-CoV-2 in blood sample suggests that the virus could not replicate in blood”, Journal of Endocrinology, Diabetes & Obesity, Vol. 9, Issue 1, 1124

14. Wenfa Ng, “High Level of Similarity in Amino Acid Sequence of Surface Proteins of SARS and SARS-Cov-2”, International Journal of Biochemistry and Physiology, Vol. 6, Issue 2, pp. 1-5, Link to article

15. Wenfa Ng, “Ammonium interference reduced copper uptake by formaldehyde crosslinked Sargassum sp. Seaweed”, Journal of Environmental Science and Public Health, Vol. 5, Issue 4, pp. 462-472, Link to article

16. Wenfa Ng, “Ribosomal proteins could explain the phylogeny of Bacillus species”, Microbiology: Current Research, Vol. 5, Issue 5, pp. 1-7, Link to article

17. Wenfa Ng, “Possible epigenetic influence on cellular differentiation and lineage segregation in Escherichia coli”, Journal of Nanotechnology Research, Accepted for publication

18. Wenfa Ng, “’Contemporary perspective on a foundational paper in role of structural maintenance of chromosome complex in mediating DNA damage response checkpoint”, Journal of Cancer Science and Clinical Therapeutics, Vol. 5, Issue 4, pp. 458-465, Link to article

19. Wenfa Ng, “High cell density cultivation of Escherichia coli DH5α in shake flasks with a new formulated medium”, Journal of Biotechnology and Bioprocessing, Vol. 2, Issue 10, Link to article

20. Wenfa Ng, “Preliminary Estimates of the Mutational Frequencies of Individual Genes in SARS-Cov-2 Genome”, Journal of Vaccines and Vaccination, Vol. 12, Issue. 6, No:1000468, Link to article

21. Wenfa Ng, “Zeta Potential of Escherichia coli DH5α Grown in Different Growth Media”, Journal of Environmental Science and Public Health, Vol. 5, Issue 4, pp. 452-461, Link to article

22. Wenfa Ng, “Effect of Polyethylene Glycol on Growth of Escherichia coli DH5α and Bacillus subtilis NRS-762”, Journal of Bioequivalence & Bioavailability, Vol. 13, Issue S5, No. 1000002, Link to article

23. Wenfa Ng, “Drastic Decline in Optical Density during Stationary Phase Meant that Bacillus subtilis NRS-762 is not Suitable as Model Organism in Microbial Survivability Studies”, Journal of Bioequivalence & Bioavailability, Vol. 13, Issue S5, No: 1000001, Link to article

24. Wenfa Ng, “Understanding the compositional changes in LB Lennox medium during growth of Escherichia coli DH5α in a 1L bioreactor”, International Journal of Applied Biology and Pharmaceutical Technology, Vol. 12, Issue 4, pp. 481-491, Link to article

25. Wenfa Ng, “Brief History of Electronic Structure Calculations in Computational Chemistry”, Journal of Nanotechnology Research, Vol. 3, Issue 1, pp. 81-91, Link to article

26. Wenfa Ng, “Ribosomal protein database profiling lends clarity to ribosomal protein evolution and mass distribution”, Journal of Bioinformatics and Systems Biology, Vol. 5, Issue 1, Link to article

27. Wenfa Ng, “Surface charge characteristics of Bacillus subtilis NRS-762 cells”, Journal of Biotechnology and Bioprocessing, Vol. 3, Issue 3, 074, Link to article

28. Wenfa Ng, “Inability to Completely Remove Trace Contaminants from Drinking Water by Adsorption”, Journal of Environmental Science and Public Health, Vol. 6, Issue 1, pp. 104-109, Link to article

29. Wenfa Ng, “Understanding Spatial and Temporal Scale in Biology”, Journal of Biotechnology and Bioprocessing, Vol. 3, Issue 3, 076, Link to article

30. Wenfa Ng, “Defining and redefining its role in biology: Synthetic biology as an emerging field at the interface of engineering and biology”, Archives of Clinical and Medical Case Reports, Vol. 6, Issue 2, pp. 224-228, Link to article

31. Wenfa Ng, “Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10”, Annals of Advanced Biomedical Sciences, Vol. 5, Issue 1, Link to article

32. Wenfa Ng, “A Short Note on Aero-Microbiology”, Applied Microbiology: Open Access, Vol. 8, Issue 2, Link to article

33. Wenfa Ng, “Phylogeny of different Helicobacter pylori strains could not be explained

by 16S rRNA gene due to high similarity in gene sequence across strains”, Journal of Microbial and Biochemical Technology, 14:493, Link to article

34. Wenfa Ng, “Application of metabolic engineering approaches in enhancing biological hydrogen production”, Journal of Genetic Engineering and Biotechnology Research, Vol. 4, Issue 2, pp. 204-207, Link to article

35. Wenfa Ng, “Relevance of Single Cell and Single Molecule Studies at Different Biological and Physical Length Scales”, Journal of Analytical Techniques and Research, Volume 4, Issue 2, Link to article

36. Wenfa Ng, “High Expression of Electron Transport Chain Genes in Blood Cells of Patient with SARS-Cov-2 Infection”, International Journal of Infectious Diseases and Research, Vol. 3, Issue 2, pp. 1-2, Link to article

37. Wenfa Ng, “Attempted fractionation of LB Lennox medium via reversed phase high performance liquid chromatography”, Journal of Chromatography and Separation Techniques, 13:484, Link to article

38. Wenfa Ng, “Technological innovations in COVID-19 diagnostics in Singapore”, Fortune Journal of Health Sciences, Vol. 6, Issue 3, pp. 267-278, Link to article

39. Wenfa Ng, “Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications”, Journal of Biotechnology and Biomedicine, Vol. 6, Issue 3, pp. 294-312, Link to article

40. Wenfa Ng, “Gastric Adenocarcinoma May Not Necessarily be Due to Helicobacter pylori or

Microbial Infection”, Archives of Clinical Microbiology, Vol. 15, No. 2: 257

Scientific research

  1. Wenfa Ng and Yen-Peng Ting, “Bacterial surface charge in “layers”: revealed by wash buffers of different ionic strength”, PeerJ Preprints, Link to preprint
  2. Wenfa Ng and Yen-Peng Ting, “Zeta potential of bacterial cells: Effect of wash buffers”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng and Yen-Peng Ting, “Formulation of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks”, PeerJ Preprints, Link to preprint
  4. Wenfa Ng, “Mouse xenograft models for elucidating resistance mechanisms to cancer therapeutics”, PeerJ Preprints, Link to preprint
  5. Wenfa Ng, “Possible odour-mediated attraction of flies to Bacillus subtilis NRS-762 stationary phase culture”, PeerJ Preprints, Link to preprint
  6. Wenfa Ng, “Graphical illustration for explaining mass spectrum fingerprinting in microbial identification”, PeerJ Preprints, Link to preprint
  7. Wenfa Ng, “Theoretical ribosomal protein mass distribution of Pseudomonas aeruginosa PAO1”, PeerJ Preprints, Link to preprint
  8. Wenfa Ng, “Conserved mass peaks in MALDI-TOF mass spectra of bacterial species at the genus and species levels”, PeerJ Preprints, Link to preprint
  9. Wenfa Ng, “Existence of theoretical ribosomal protein mass fingerprints in bacteria, archaea and eukaryotes”, PeerJ Preprints, Link to preprint
  10. Wenfa Ng, “Ammonium interference reduced copper uptake by formaldehyde-crosslinked Sargassum seaweed”, PeerJ Preprints, Link to preprint
  11. Wenfa Ng, “Colourless agar for enhancing colour contrast between microbial colonies and agar”, PeerJ Preprints, Link to preprint
  12. Wenfa Ng, “High cell density cultivation of Escherichia coli DH5α in shake flasks with a new formulated medium”, PeerJ Preprints, Link to preprint
  13. Wenfa Ng, “Surface charge characteristics of Bacillus subtilis NRS-762 cells”, PeerJ Preprints, Link to preprint
  14. Wenfa Ng, “Zeta potential of Escherichia coli DH5α grown in different growth media”, PeerJ Preprints, Link to preprint
  15. Wenfa Ng, “Microbes in deionized and tap water: Implications for maintenance of laboratory water production system”, PeerJ Preprints, Link to preprint
  16. Wenfa Ng, “Polyethylene glycol exerted toxicity to growth of Bacillus subtilis NRS-762”, PeerJ Preprints, Link to preprint
  17. Wenfa Ng, “Drastic decline in optical density during stationary phase meant that Bacillus subtilis NRS-762 is not suitable as model organism in microbial survivability studies”, PeerJ Preprints, Link to preprint
  18. Wenfa Ng, “Theoretical ribosomal protein mass fingerprint database” PeerJ Preprint, Link to preprint
  19. Wenfa Ng, “Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10”, PeerJ Preprints, Link to preprint
  20. Wenfa Ng, “Common mistakes in Western blot”, figshare, Link to preprint
  21. Wenfa Ng, “Number of mismatches and length of longest match correlate with alignment score in swalign built-in function in MATLAB”, bioRxiv, Link to preprint
  22. Wenfa Ng, “Annotation of ribosomal protein mass peaks in MALDI-TOF mass spectra of bacterial species and their phylogenetic significance”, bioRxiv, Link to preprint
  23. Wenfa Ng, “Ribosomal proteins could explain the phylogeny of Bacillus species”, bioRxiv, Link to preprint
  24. Wenfa Ng, “Effect of polyethylene glycol on growth of Escherichia coli DH5α and Bacillus subtilis NRS-762”, bioRxiv, Link to preprint
  25. Wenfa Ng, “ICP-MS analysis of metal and metalloid concentrations of common microbiological growth media reveals presence of heavy metals”, bioRxiv, Link to preprint
  26. Wenfa Ng, “High level of similarity in amino acid sequence of surface proteins of SARS and SARS-CoV-2”, figshare, Link to preprint
  27. Wenfa Ng, “Analysis on the sample types compatible with different types of SARS-CoV-2 diagnostic test”, figshare, Link to preprint
  28. Wenfa Ng, “Developing an ethanol utilisation pathway based NADH regeneration system in Escherichia coli”, bioRxiv, Link to preprint
  29. Wenfa Ng, “ORF10 offers a unique target for design of primers for detecting SARS-CoV-2 through RT-qPCR”, figshare, Link to preprint
  30. Wenfa Ng, “Lack of mRNA transcripts of SARS-CoV-2 in blood sample suggests that the virus could not replicate in blood”, figshare, Link to preprint
  31. Wenfa Ng, “High expression of electron transport chain genes in blood cells of patient with SARS-CoV-2 infection”, figshare, Link to preprint
  32. Wenfa Ng, “SARS-CoV-2 infection does not necessarily lead to co-infection of blood with other microbial species”, figshare, Link to preprint
  33. Wenfa Ng, “Preliminary estimates of the mutational frequencies of individual genes in SARS-CoV-2 genome”, figshare, Link to preprint
  34. Wenfa Ng, “Gastric adenocarcinoma may not necessarily be due to Helicobacter pylori or microbial infection”, figshare, Link to preprint
  35. Wenfa Ng, “Phylogenetic tree of Helicobacter species”, figshare, Link to preprint
  36. Wenfa Ng, “Confluence of factors that enable technology to play a larger role in diagnosis of SARS-CoV-2”, figshare, Link to preprint
  37. Wenfa Ng, “Overview of diagnostic tests developed to detect SARS-CoV-2 infection”, figshare, Link to preprint
  38. Wenfa Ng, “Employing Statistical Machine Reading for Inferring Key Concepts of a Research Field From a Body of Abstracts and Blog Posts”, Preprints, Link to preprint
  39. Wenfa Ng, “Ribosomal protein database profiling lends clarity to ribosomal protein evolution and mass distribution”, bioRxiv, Link to preprint
  40. Wenfa Ng, “Categorization of prophage genes in Bacillus subtilis 168 and assessing their relative importance through RNA-seq gene expression analysis”, bioRxiv, Link to preprint
  41. Wenfa Ng, “26S rRNA gene is not useful for identifying Saccharomycodes ludwigii by qPCR”, figshare, Link to preprint
  42. Wenfa Ng, “Good expression of cpsADH in recombinant Escherichia coli cultivated in LB and M9 ethanol medium”, figshare, Link to preprint
  43. Wenfa Ng, “Development of protocol for dry heat inactivation of Escherichia coli DH5α”, figshare, Link to preprint
  44. Wenfa Ng, “Phylogeny of different Helicobacter pylori strains could not be explained by 16S rRNA gene due to high similarity in gene sequence across strains”, Research Square, Link to preprint
  45. Wenfa Ng, “Database of small proteins of fewer than 50 amino acids in different microbial species”, SSRN, Link to preprint
  46. Wenfa Ng, “Database of endogenized phage genes of different microbial species”, SSRN, Link to preprint
  47. Wenfa Ng, “Gene expression database of different microbial species”, SSRN, Link to preprint
  48. Wenfa Ng, “Illustrative protocol for determining the centrifugation parameters of bacterial cells”, SSRN, Link to preprint
  49. Wenfa Ng, “Development of a proteome database of prokaryotic and eukaryotic microorganisms”, SSRN, Link to preprint
  50. Wenfa Ng, “Statistical analysis of number of genes and chromosome lengths of different microbial species”, bioRxiv, Link to preprint
  51. Wenfa Ng, “No endogenized phage gene in Helicobacter pylori genome”, figshare, Link to preprint
  52. Wenfa Ng, “Background fluorescence in qPCR assay decrease with cycle number at the start of the assay prior to amplicon amplification”, figshare, Link to preprint
  53. Wenfa Ng, “Annotation of the protocol for preparing chemically competent cells”, BioRN, Link to preprint
  54. Wenfa Ng, “Possible degradation of plasmid in Escherichia coli DH5α stored at 4 oC”, BioRN, Link to preprint
  55. Wenfa Ng, “In silico bioinformatics analysis reveals that shotgun metagenomics provides highly accurate identification of different microorganisms”, Manuscript in preparation
  56. Wenfa Ng, “Escherichia coli cells stored at 4 oC could be used as inoculum to activate a microbial culture”, BioRN, Link to preprint
  57. Wenfa Ng, “No microbial ribosomal protein transcripts found in normal and neoplasm in human gastric adenocarcinoma”, figshare, Link to preprint
  58. Wenfa Ng, “Public electron micrographs suggest interconnecting filaments in Helicobacter pylori cells that may serve as conduits for genetic transfer”, figshare, Link to preprint
  59. Wenfa Ng, “Theoretical 2D protein gel of Helicobacter pylori”, Research Square, Link to preprint
  60. Wenfa Ng, “Annotation of Helicobacter pylori mass peaks detected by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry”, Research Square, Link to preprint
  61. Wenfa Ng, “Theoretical mass spectrum of Helicobacter pylori”, Research Square, Link to preprint
  62. Wenfa Ng, “Brief descriptions of operating principles and mechanisms in major types of COVID-19 diagnostics”, figshare, Link to preprint
  63. Wenfa Ng, “Shear stress likely reduced viability of Escherichia coli cells as they pass through a narrow constriction in PMMA microfluidic chip”, Research Square, Link to preprint
  64. Wenfa Ng, “Distribution of number of genes in different bacteriophages”, Research Square, Link to preprint
  65. Wenfa Ng, “Theoretical analysis suggests that variants of 16S rRNA gene of Helicobacter pylori could not be differentiated by PCR melt curve analysis of amplicons”, Research Square, Link to preprint
  66. Wenfa Ng, “Simulated sequencing of Helicobacter pylori 16S rRNA gene reveals varied sequence length indicative of splicing events”, SSRN, Link to preprint
  67. Wenfa Ng, “Technological Innovations in COVID-19 Diagnostics in Singapore”, Preprints, Link to preprint
  68. Wenfa Ng, “Understanding the Different Parameters That Affects Bacterial Transformation Efficiency”, Preprints, Link to preprint
  69. Wenfa Ng, “Metrics for Understanding the Degraded Metagenome of Stomach Cancer Patient”, Preprints, Link to preprint
  70. Wenfa Ng, “Recent Developments in High Spatio-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications”, Preprints, Link to preprint
  71. Wenfa Ng, “Phylogeny of Bacillus subtilis strains cannot be resolved by 16S rRNA due to very close evolutionary distance in the gene”, SSRN, Link to preprint
  72. Wenfa Ng, “Extremely low expression of ribosomal proteins support observed slow physiological growth rate of Helicobacter pylori”, SSRN, Link to preprint
  73. Wenfa Ng, “Assessing the species identification efficiency of different 16S rRNA gene universal primers for stomach amplicon metagenomics”, SSRN, Link to preprint
  74. Wenfa Ng, “Single phase biotransformation of acetophenone to 1-phenylethanol using recombinant Escherichia coli expressing cpsADH alcohol dehydrogenase”, Research Square, Link to preprint
  75. Wenfa Ng, “Proposed partial disease model for SARS-CoV-2 infection”, figshare, Link to preprint
  76. Wenfa Ng, “Case example of no alignment of microbial ribosomal protein genes from whole blood mRNA transcripts of COVID-19 positive patient”, figshare, Link to preprint
  77. Wenfa Ng, “Candidate microRNA therapeutics for genes in the SARS-CoV-2 genome”, figshare, Link to preprint
  78. Wenfa Ng, “Difficulty in Achieving High Identification Accuracy and High Amplification Efficiency in Bacterial 16s rRNA Amplicon Metagenomics”, Preprints, Link to preprint
  79. Wenfa Ng, “Lack of correlation between gel band intensity and DNA concentration”, figshare, Link to preprint
  80. Wenfa Ng, “Preliminary findings of kanamycin resistant microbes in deionized water in an agar culture survey”, SSRN, Link to preprint
  81. Wenfa Ng, “Relative lack of diversity of microbes recovered on LB Miller agar medium”, Research Square, Link to preprint
  82. Wenfa Ng, “Initial work exploring ethylene glycol utilization in Bacillus subtilis”, figshare, Link to preprint
  83. Wenfa Ng, “Experimental work on engineering better NADH regeneration with ethanol utilization pathway”, figshare, Link to preprint
  84. Wenfa Ng, “Conversion efficiency saturates at high substrate concentration in acetophenone to 1-phenylethanol two-phase whole-cell biocatalysis”, SSRN, Link to preprint
  85. Wenfa Ng, “Examining the kinetics of E. coli whole cell biotransformation of acetophenone to 1-Phenylethanol”, SSRN, Link to preprint
  86. Wenfa Ng, “No tight correlation between transcription and translation for highly expressed genes in Pseudomonas fluorescens”, Research Square, Link to preprint
  87. Wenfa Ng, “Emergence of small amount of organic leaching during copper sorption on treated seaweed under ammonium interference”, Research Square, Link to preprint
  88. Wenfa Ng, “Partial re-analysis of upper respiratory tract microbiome of COVID-19 positive patient reveals diverse microbial species”, figshare, Link to preprint
  89. Wenfa Ng, “Dataset of DNA microarray probe for every gene in the genome of Escherichia coli K-12”, figshare, Link to dataset
  90. Wenfa Ng, “Effect of template and primer-probe concentration level on shape of qPCR amplification curve”, Research Square, Link to preprint
  91. Wenfa Ng, “Catalogue of secretion systems in different bacterial species”, SSRN, Link to preprint
  92. Wenfa Ng, “Streptococcus salivarius may have a good acid efflux system to protect its genome from acid attack”, figshare, Link to preprint
  93. Wenfa Ng, “Detection of Two Phylogenetic Clusters in Helicobacter pylori 23S rRNA Phylogeny Suggests Emergence of Two Distinct Phylotype of the Species”, Preprints, Link to preprint
  94. Wenfa Ng, “An alternative mechanism for climate change due to rising atmospheric carbon dioxide concentration”, SSRN, Link to preprint
  95. Wenfa Ng, “Selective dissolution and precipitation for mechanical recycling of post-use plastics”, Archive.org, Link to preprint
  96. Wenfa Ng, “Review of genetics-free high-throughput approach for activating production of cryptic microbial secondary metabolites”, Archives.org, Link to preprint
  97. Wenfa Ng, “Automated screening of DNA sequence of gene for recognition sequence of specific restriction enzymes”, Archives.org, Link to preprint
  98. Wenfa Ng, “Possible cannibalism in Bacillus subtilis NRS-762 stationary phase culture”, Archives.org, Link to preprint
  99. Wenfa Ng, “Review of using diacetic functionalized polymers in solubilizing enzymes in organic solvents”, Archives.org, Link to preprint
  100. Wenfa Ng, “Photo of lichen on tree: Caution on identifying microorganisms based on photographs”, Archives.org, Link to preprint
  101. Wenfa Ng, “Growth of Bacillus subtilis NRS-762, Pseudomonas aeruginosa PRD-10, Pseudomonas protegens Pf-5, Escherichia coli DH5α, and Chromobacterium violaceum on LB Lennox agar”, Archives.org, Link to preprint
  102. Wenfa Ng, “Examining the possibility of expressing hydrogenase under aerobic conditions in Escherichia coli”, Archives.org, Link to preprint
  103. Wenfa Ng, “Analysis on the sample types compatible with different types of SARS-CoV-2 diagnostic test”, Archives.org, Link to preprint
  104. Wenfa Ng, “Confluence of factors that enable technology to play a larger role in diagnosis of SARS-CoV-2”, Archives.org, Link to preprint
  105. Wenfa Ng, “Gastric adenocarcinoma may not necessarily be due to Helicobacter pylori or microbial infection”, Archives.org, Link to preprint
  106. Wenfa Ng, “Brief descriptions of operating principles and mechanisms in major types of COVID-19 diagnostics”, Archives.org, Link to preprint

Education research

  1. Wenfa Ng, “Bacterial calligraphy: A memento for undergraduate research students”, Journal of Microbiology and Biology Education, Vol. 13, No. 2, pp. 172-174, Link to article
  2. Wenfa Ng, “Teaching microbial identification with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and bioinformatics tools”, Journal of Microbiology and Biology Education, Vol. 14, No. 1, pp. 103-106, Link to article
  3. Wenfa Ng, “Preprints as medium for teaching science writing”, figshare, Link to article
  4. Wenfa Ng, “Developing a theme for a tutorial set facilitates learning of concepts”, figshare, Link to article
  5. Wenfa Ng, “Standardized marker size and line width helps reduce distorted presentation of graphs to readers”, figshare, Link to article
  6. Wenfa Ng, “Teaching students to analyze issues from different perspectives through constructing narratives of electron micrographs”, figshare, Link to preprint
  7. Wenfa Ng, “Discussing policy underpinnings and implications of regulating carbon dioxide emissions for introducing technology policy considerations to engineering students”, figshare, Link to preprint
  8. Wenfa Ng, “Socratic approach for unlocking creative potential in undergraduate research students”, PeerJ Preprints, Link to preprint
  9. Wenfa Ng, “Possible topics for a course in cancer biology”, figshare, Link to preprint
  10. Wenfa Ng, “Possible topics for a course on biostatistics”, figshare, Link to preprint
  11. Wenfa Ng, “Possible topics for a course on techniques for biomedical research”, figshare, Link to preprint
  12. Wenfa Ng, “The value of a honours degree is in educating an individual to tackle problems in life”, figshare, Link to preprint
  13. Kwi-Shan Seah, and Wenfa Ng, “Reflections on a lab rotation integrating science writing and experiment work”, figshare, Link to preprint
  14. Wenfa Ng, “Possible topics for a course on Methods and Approaches in Quantitative Biology”, figshare, Link to preprint
  15. Wenfa Ng, “Possibility of using clinical photo quiz to teach detective medical inquiry in undergraduate medical school”, figshare, Link to preprint
  16. Wenfa Ng, “Possible topics for a course on Quantitative Physiology”, SSRN, Link to preprint

Citizen science mini-projects

  1. Wenfa Ng, “Citizen science mini-project: Learning the structure of scientific names”, figshare, Link to preprint
  2. Wenfa Ng, “Citizen science mini-project: Understanding shapes of microbes through electron micrographs”, figshare, Link to preprint
  3. Wenfa Ng, “Citizen science mini-project: Understanding the structural basis of protein function”, figshare, Link to preprint
  4. Wenfa Ng, “Citizen science mini-project: Gleaning useful insights about a microbe from its Wikipedia webpage”, figshare, Link to preprint
  5. Wenfa Ng, “Citizen science mini-project: Know your microbe through its 16S rRNA gene sequence”, figshare, Link to preprint
  6. Wenfa Ng, “Citizen science mini-project: Deciphering a microbial growth curve”, SSRN, Link to preprint

Hypothesis

  1. Wenfa Ng, “Defining the Mutational and Conformational Space of Prokaryotic Ribosomes for Enhanced Function”, Preprints, Link to preprint
  2. Wenfa Ng, “Exploring the biotechnological potential of C2 alternative substrates in biocatalysis and metabolic engineering”, figshare, Link to preprint

Perspectives

  1. Wenfa Ng, “Cost hurdles to open access publishing: A citizen scientist’s perspective”, PeerJ Preprints, Link to preprint
  2. Wenfa Ng, “Defining and redefining its role in biology: Synthetic biology as an emerging field at the interface of engineering and biology”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng, “Preprints as medium for communicating new ideas, hypotheses, data, analysis and beyond”, PeerJ Preprints, Link to preprint
  4. Wenfa Ng, “Relevance of single cell and single molecule studies at different biological and physical length scales”, PeerJ Preprints, Link to preprint
  5. Wenfa Ng, “Understanding spatial and temporal scale in biology”, figshare, Link to preprint
  6. Wenfa Ng, “Material and design challenges in phase change memory”, Preprints, Link to preprint
  7. Wenfa Ng, “What drives computational chemistry forward: theory or computational power?”, Preprints, Link to preprint
  8. Wenfa Ng, “Metabolism of Non-growing Cells and Their Application in Biotransformation”, Preprints, Link to preprint
  9. Wenfa Ng, “Application of metabolic engineering approaches in enhancing biological hydrogen production”, Preprints, Link to preprint
  10. Wenfa Ng, “Production of coenzyme Q10 in recombinant Schizosaccharomyces pombe through metabolic engineering approaches”, figshare, Link to preprint
  11. Wenfa Ng, “Evaluating the potential of applying machine learning tools to metabolic pathway optimization”, Preprints, Link to preprint
  12. Wenfa Ng, “Possibility of Tapping NADH Regenerated from Ethylene Glycol Utilization Pathway for Cofactor Regeneration”, Preprints, Link to preprint
  13. Wenfa Ng, “Possibility of teaching Bacillus subtilis ethylene glycol utilization”, figshare, Link to preprint
  14. Wenfa Ng, “Examining how different carbon entry point affects recombinant protein production from ethylene glycol in Bacillus subtilis”, figshare, Link to preprint
  15. Wenfa Ng, “Predicting regulatory motifs of enzymes using machine learning”, figshare, Link to preprint
  16. Kang Zhou, Wenfa Ng, Yoel Cortés-Peña, and Wang Xiaonan, “Increasing metabolic pathway flux by using machine learning models”, Current Opinion in Biotechnology, Vol. 66, pp. 179-185, Link to article
  17. Wenfa Ng, “Evaluating potential of ethanol-based NADH regeneration in supporting whole-cell biocatalysis”, figshare, Link to preprint
  18. Wenfa Ng, “Harnessing residual material and energy value from waste plastics”, figshare, Link to preprint
  19. Wenfa Ng, “Brief History of Electronic Structure Calculations in Computational Chemistry”, figshare, Link to preprint

Ideas

  1. Wenfa Ng, “Splice variants’ role in mediating different disease states in tissue requiring ion transduction through calcium channel for function”, PeerJ Preprints, Link to preprint
  2. Wenfa Ng, “Possible variation in microbiota community structure and composition in same species bred in zoos and a wildlife refuge, and its implications on reproductive success”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng, “Poleward and vertical migration of animals under climate change highlights possible environmental effects on social behavior”, PeerJ Preprints, Link to preprint
  4. Wenfa Ng, “Transparent PET optical window for microfluidic cell culture device”, PeerJ Preprints, Link to preprint
  5. Wenfa Ng, “Average deviation for measuring variation in data in small samples (n < 5)”, PeerJ Preprints, Link to preprint
  6. Wenfa Ng, “Single cell transcriptomics view of cell lineage, cell fate and cellular differentiation”, PeerJ Preprints, Link to preprint
  7. Wenfa Ng, “Avoiding CRISPR: Plasmid design for genetic engineering”, PeerJ Preprints, Link to preprint
  8. Wenfa Ng, “High mass resolution MALDI-TOF MS for profiling biomolecules in mixtures”, PeerJ Preprints, Link to preprint
  9. Wenfa Ng, “Type II diabetes may affect stem cell niche resulting in down regulation of glucose transporters and insulin receptors in cells”, PeerJ Preprints, Link to preprint
  10. Wenfa Ng, “Difficulty in dislodging biofilms from inner surface of drinking water pipelines”, PeerJ Preprints, Link to preprint
  11. Wenfa Ng, “Blood biopsy profiles circulating tumour cells which hold clues to cancer treatment”, PeerJ Preprints, Link to preprint
  12. Wenfa Ng, “Elaborate mechanism for the synthesis of lipopolysaccharide points to its functional importance in Gram-negative bacteria”, PeerJ Preprints, Link to preprint
  13. Wenfa Ng, “Possibility of correlating mutational profile of cancer with drugs suitable for treatment”, PeerJ Preprints, Link to preprint
  14. Wenfa Ng, “Bacterial Type VI secretion system could have evolved from co-opted tail sheath tube of bacteriophages”, PeerJ Preprints, Link to preprint
  15. Wenfa Ng, “Possibility of detecting cancer using liquid chromatography mass spectrometry profiling of cancer signature metabolome in blood”, PeerJ Preprints, Link to preprint
  16. Wenfa Ng, “Search for receptors in immune cells that bind cancer cell antigens and their activation in silent cases”, PeerJ Preprints, Link to preprint
  17. Wenfa Ng, “Snippets of virus integrated into human genome suggests possibility of CRISPR-like adaptive immune system in human cells”, PeerJ Preprints, Link to preprint
  18. Wenfa Ng, “Accessing the metabolism of viable but non-culturable (VBNC) microbes”, PeerJ Preprints, Link to preprint
  19. Wenfa Ng, “Merits of constant expression of CRISPR loci in adaptive immunity of bacteria”, PeerJ Preprints, Link to preprint
  20. Wenfa Ng, “Possible differences in efficiency of guide RNA with different spacer sequence in CRISPR knock-down or knock-out of particular gene”, PeerJ Preprints, Link to preprint
  21. Wenfa Ng, “Possible epigenetic influence on cellular differentiation and lineage segregation in Escherichia coli”, PeerJ Preprints, Link to preprint
  22. Wenfa Ng, “Biochemical traits that Escherichia coli evolved during its adaption to the gastrointestinal tract of humans”, PeerJ Preprints, Link to preprint
  23. Wenfa Ng, “In silico experiments validate that twenty nucleotide spacer sequence provides precise targeting of bacterial genes in CRISPR-Cas9”, PeerJ Preprints, Link to preprint
  24. Wenfa Ng, “Exploring lowering the optimal growth temperature of Escherichia coli in biotechnology applications”, PeerJ Preprints, Link to preprint
  25. Wenfa Ng, “Possibility of understanding metabolic syndrome by probing dysregulation of metabolic and signaling network in bacteria”, PeerJ Preprints, Link to preprint
  26. Wenfa Ng, “Possibility of tuning the differentiation state of tumor associated macrophages towards tumor controlling phenotypes”, PeerJ Preprints, Link to preprint
  27. Wenfa Ng, “Understanding gene transfer between endosymbiotic organisms”, PeerJ Preprints, Link to preprint
  28. Wenfa Ng, “Understanding the chemical basis for the preferential ionization of specific biomolecules in mass spectrometry analysis of microbial cells”, PeerJ Preprints, Link to preprint
  29. Wenfa Ng, “Proposal of conceptual model for the mechanism underpinning evolution of operon as pathway gene organizer in bacterial genome”, PeerJ Preprints, Link to preprint
  30. Wenfa Ng, “Understanding the evolutionary origins of bacterial natural products with immunosuppressant properties”, PeerJ Preprints, Link to preprint
  31. Wenfa Ng, “Cultivating the uncultivable environmental microbe using insights from whole genome sequence that define a growth medium”, PeerJ Preprints, Link to preprint
  32. Wenfa Ng, “Could analysis of biofilm formation and swarming motility in Pseudomonas aeruginosa inform evolution of multicellular behaviour in mammalian system?”, figshare, Link to preprint
  33. Wenfa Ng, “Analyzing microbial whole cell mass spectrum obtained using MALDI-TOF MS not-dedicated for microbial identification”, figshare, Link to preprint
  34. Wenfa Ng, “Evolution of specific codon usage patterns in particular microbial species”, figshare, Link to preprint
  35. Wenfa Ng, “Applying machine learning tools to glean complex regulatory networks in metabolism”, figshare, Link to preprint
  36. Wenfa Ng, “Application of machine learning to understand biophysical rules that govern protein folding and activity”, figshare, Link to preprint
  37. Wenfa Ng, “Determining possible redundancy in metabolic pathways of a microorganism”, figshare, Link to preprint
  38. Wenfa Ng, “Possibility in using computational screening of binding partners of therapeutic molecules to identify potential side effects of drugs”, figshare, Link to preprint
  39. Wenfa Ng, “Engineering subcellular microcompartments with molecular sieve properties to facilitate compartmentalized metabolic engineering in bacteria”, figshare, Link to preprint
  40. Wenfa Ng, “Feasibility of modulating metabolite secretion in microbial production host in easing demands on downstream purification in bioprocessing”, figshare, Link to preprint
  41. Wenfa Ng, “Subfield citation index describes the influence of a paper compared to its immediate surrounding field”, figshare, Link to preprint
  42. Wenfa Ng, “Deactivation of reversible enzyme function through rational protein engineering”, figshare, Link to preprint
  43. Wenfa Ng, “Identification of enzymes that lack a substrate through bioinformatic search for lack of sequence homology”, figshare, Link to preprint
  44. Wenfa Ng, “Batteries as energy storage nexus connecting solar power with grid power for stable provision of electricity”, figshare, Link to preprint
  45. Wenfa Ng, “Elliptical orbit of 2003 VB12 (Sedna) suggests possible orbit around dark matter center with the solar system as counter mass”, figshare, Link to preprint
  46. Wenfa Ng, “Would fluorescent labelling affect cancer cell metastasis?”, figshare, Link to preprint
  47. Wenfa Ng, “Trade-offs between energy efficiency and indoor air quality”, figshare, Link to preprint
  48. Wenfa Ng, “City scale wind profile map for understanding distribution of urban air pollutants’ levels and responding to severe weather events like wind stagnation and smog”, figshare, Link to preprint
  49. Wenfa Ng, “Solar intensity map of a city helps guide the deployment of solar power generative capacity”, figshare, Link to preprint
  50. Wenfa Ng, “Defining the mutational and conformational space of prokaryotic ribosomes”, figshare, Link to preprint
  51. Wenfa Ng, “Brief history of computational chemistry: Three distinct eras and the relative importance of theoretical insights and computing power in advancing the field”, PeerJ Preprints, Link to preprint
  52. Wenfa Ng, “Towards extracellular secretion of poly-β-hydroxybutyric acid (PHB) biodegradable polymer”, PeerJ Preprints, Link to preprint
  53. Wenfa Ng, “Understanding the formation of water droplets on microbial cell surface under a variety of humidity conditions”, figshare, Link to preprint
  54. Wenfa Ng, “Determining the growth phase of cells in a microbial colony on solid agar”, figshare, Link to preprint
  55. Wenfa Ng, “Development of a stimuli-responsive microcapsule capable of recognizing and killing cancer cells through mechanical actuation”, figshare, Link to preprint
  56. Wenfa Ng, “Possibility of using long read sequencing for analysing the mRNA transcriptome of cells”, figshare, Link to preprint
  57. Wenfa Ng, “Presence of large number of highly expressed transposase and transposase helper genes in the genome of Pseudomonas syringae DC3000”, figshare, Link to preprint
  58. Wenfa Ng, “Understanding the energy landscape that guides protein folding using machine learning and molecular simulations”, figshare, Link to preprint
  59. Wenfa Ng, “RNA-seq could detect single nucleotide polymorphism in coding genes”, figshare, Link to preprint
  60. Wenfa Ng, “What drove bacterial cells to adopt an intracellular lifestyle in eukaryotic cells during pathogenesis?”, figshare, Link to preprint
  61. Wenfa Ng, “Using the human gut microbiome as inoculum for anaerobic digestion of food waste to produce amino acids and useful metabolites”, figshare, Link to preprint
  62. Wenfa Ng, “Determining the minimum temperature at which bacterial cells could grow”, figshare, Link to preprint
  63. Wenfa Ng, “Building a minimal bacterial cell for biotechnology applications”, figshare, Link to preprint
  64. Wenfa Ng, “Protein degradation is one of two switches that control gene expression”, figshare, Link to preprint
  65. Wenfa Ng, “Bioinformatic identification of prion proteins sequence in the genome of Escherichia coli and other bacterial species”, figshare, Link to preprint
  66. Wenfa Ng, “In situ sequencing is a modern incarnation of DNA microarray differential gene expression analysis”, figshare, Link to preprint
  67. Wenfa Ng, “Substrate utilization profile could inform the characteristics of the promoter controlling expression of genes involved in substrate catabolism”, figshare, Link to preprint
  68. Wenfa Ng, “Possibility of adding a DNA amplification step to single cell RNA sequencing workflow”, figshare, Link to preprint
  69. Wenfa Ng, “Understanding factors responsible for stable maintenance of diverse biosynthetic gene clusters in different organisms”, figshare, Link to preprint
  70. Wenfa Ng, “Reversibility in enzymes belonging to central carbon metabolism endows an evolutionary fitness in enabling ability to grow on different substrates”, figshare, Link to preprint
  71. Wenfa Ng, “Using mutational signature of each cancer type to develop multiplex sequencing tools for early blood-based screening of cancer”, figshare, Link to preprint
  72. Wenfa Ng, “Understanding the spatial localisation of gene product transcribed from a mRNA encoding an operon in a bacterial cell”, figshare, Link to preprint
  73. Wenfa Ng, “Adding energy calculations may help machine learning better predict protein structures”, figshare, Link to preprint
  74. Wenfa Ng, “Metabolism in bacterial species may not be optimized”, figshare, Link to preprint
  75. Wenfa Ng, “Genome sequencing and metabolic network reconstruction offers a faster peek into microbial metabolism and augments metabolomics research”, figshare, Link to preprint
  76. Wenfa Ng, “Reducing carbon loss is a major theme in rewiring of endogenous metabolic pathways”, figshare, Link to preprint
  77. Wenfa Ng, “Predicting disease susceptibility and propensity of metabolic dysfunction from relative abundances of enzymes inferred from RNA-seq data of humans”, figshare, Link to preprint
  78. Wenfa Ng, “Homology-based structural analysis of ribosomal proteins in Bacillus subtilis”, figshare, Link to preprint
  79. Wenfa Ng, “Heterologous expression of “food protein” in Chlorella vulgaris for generating a microalgal based protein factory”, figshare, Link to preprint
  80. Wenfa Ng, “Determining the inducers of autoinducible promoters detected in RNA-seq transcriptomic profiling”, figshare, Link to preprint
  81. Wenfa Ng, “Viral polyproteins offer expeditious protein translation that shorten the viral life cycle and culminates with greater transmissibility”, figshare, Link to preprint
  82. Wenfa Ng, “Possibility of simulating the folding of proteins as it is translated from the ribosome”, figshare, Link to preprint
  83. Wenfa Ng, “Possibility of informing drug choices based on insights from the human genome”, figshare, Link to preprint
  84. Wenfa Ng, “Understanding how microbes with differing oxygen tolerance assemble as microbiome in a model gastrointestinal lining on a chip device”, figshare, Link to preprint
  85. Wenfa Ng, “Differential gene expression analysis through RNA-seq typically identifies correlations which must be validated for causality”, figshare, Link to preprint
  86. Wenfa Ng, “Determining the genetic regulon of different nutrients that a bacterial cell process”, figshare, Link to preprint
  87. Wenfa Ng, “RNA-seq transcriptome informs the relative abundance of different enzymes in central carbon metabolism”, figshare, Link to preprint
  88. Wenfa Ng, “Building a theoretical enzyme model could facilitate screening of Protein Data Bank for uncovering existing enzyme candidates ripe for reaction repurposing”, figshare, Link to preprint
  89. Wenfa Ng, “RNA-seq transcriptome informs the genes slated for over-expression or deletion in metabolic engineering”, figshare, Link to preprint
  90. Wenfa Ng, “Rational design of a growth medium capable of allowing gastrointestinal enteric bacteria to mimic growth in the human gut environment”, figshare, Link to preprint
  91. Wenfa Ng, “Observations of transcriptional burst of ribosomal proteins in Bacillus cereus ATCC 10987 during first twenty minutes of cultivation”, figshare, Link to preprint
  92. Wenfa Ng, “Stationary phase in bacterial culture does not imply low overall expression level for genes”, figshare, Link to preprint
  93. Wenfa Ng, “Computational protein structure prediction coupled with atomistic molecular dynamics docking experiments afford glimpse of ancient events in protein structural evolution”, figshare, Link to preprint
  94. Wenfa Ng, “Enhanced biomass formation of Escherichia coli DH5α in formulated medium with yeast extract as secondary carbon and nitrogen source”, figshare, Link to preprint
  95. Wenfa Ng, “Examining the question why glucose is the preferred sugar for cellular metabolism from the phylogenetic perspective”, figshare, Link to preprint
  96. Wenfa Ng, “Glucose preferred status as substrate may hint on its early existence in the origins of life on Earth”, figshare, Link to preprint
  97. Wenfa Ng, “Concept of strong or weak connection could be used to describe the size of metabolic flux that could be diverted to an intermediate from another pathway”, figshare, Link to preprint
  98. Wenfa Ng, “Expressing Bacillus subtilis sporulation complex genes in Escherichia coli”, figshare, Link to preprint
  99. Wenfa Ng, “RNA-seq transcriptomic analysis reveals that ribosomal proteins are not the most highly expressed proteins”, figshare, Link to preprint
  100. Wenfa Ng, “Subunits of an enzyme complex are typically not expressed in stoichiometric proportion”, figshare, Link to preprint
  101. Wenfa Ng, “Targeting master regulators in cancer treatment may incur side effects”, figshare, Link to preprint
  102. Wenfa Ng, “Processing larger number of sequence reads increases the probability of detecting RNA transcripts from low expression genes”, figshare, Link to preprint
  103. Wenfa Ng, “Genes that are not expressed could be candidates as locus for integration of heterologous operon”, figshare, Link to preprint
  104. Wenfa Ng, “Glycolysis is the metabolic highway that connects different carbon substrates to central carbon metabolism”, figshare, Link to preprint
  105. Wenfa Ng, “Glyoxylate cycle is not essential for supporting aerobic growth in bacterial species”, figshare, Link to preprint
  106. Wenfa Ng, “Carbon dioxide gas hydrate formation is water intensive and incurs an environmental and energy footprint”, figshare, Link to preprint
  107. Wenfa Ng, “Understanding the biological basis of MALDI-TOF mass spectrometry-based microbial identification”, figshare, Link to preprint
  108. Wenfa Ng, “Carbon rearrangement reactions may have evolved to produce new intermediates while conserving total number of carbon atoms in molecules”, figshare, Link to preprint
  109. Wenfa Ng, “Utility of atomistic simulations in constraining mechanisms of chemical reactions”, figshare, Link to preprint
  110. Wenfa Ng, “Examining the possibility of expressing hydrogenase under aerobic conditions in Escherichia coli”, figshare, Link to preprint
  111. Wenfa Ng, “Probing co-evolution of ribosomal proteins through structural phylogeny”, figshare, Link to preprint
  112. Wenfa Ng, “Small genome encoding fewer genes calls for a fundamental rethink of Archaea role in the evolution of microbial life on Earth”, figshare, Link to preprint
  113. Wenfa Ng, “Archaea may have been outcompeted by bacteria and evolved to grow in extreme environments”, figshare, Link to preprint
  114. Wenfa Ng, “Bacillus subtilis could repair nick in plasmid assembled by ligation independent cloning”, figshare, Link to preprint
  115. Wenfa Ng, “Bacillus subtilis could not readily utilize organic sulphur for growth”, figshare, Link to preprint
  116. Wenfa Ng, “Bacillus subtilis could not be used to produce more plasmid DNA during molecular cloning”, figshare, Link to preprint
  117. Wenfa Ng, “Plasmids with pMB1 replication origin are likely of low copy number in Bacillus subtilis”, figshare, Link to preprint
  118. Wenfa Ng, “High biomass formation capacity and lack of metabolic defects should be essential criteria in selecting a microbial chassis for biotechnology applications”, figshare, Link to preprint
  119. Wenfa Ng, “PthrC and PgyrA promoter could not be used for heterologous gene expression in Bacillus subtilis”, figshare, Link to preprint
  120. Wenfa Ng, “Promoter controlling acetyl-CoA synthetase in Bacillus subtilis may be inducible by acetate”, figshare, Link to preprint
  121. Wenfa Ng, “Resting cells likely retain gene expression activity for keeping cells viable”, figshare, Link to preprint
  122. Wenfa Ng, “Expressing Bacillus subtilis sporulation complex genes in Escherichia coli”, figshare, Link to preprint
  123. Wenfa Ng, “Scanning electron microscopy images of autoclaved Escherichia coli cells suggest intact cells after treatment”, figshare, Link to preprint
  124. Wenfa Ng, “Glucose may help up-regulate expression of needed enzymes and pathways in central carbon metabolism during utilization of unconventional substrates”, figshare, Link to preprint
  125. Wenfa Ng, “Scope of a research field evolves as the field develops”, figshare, Link to preprint
  126. Wenfa Ng, “Reliance on annotated genes and pathways meant that much of metabolic engineering research still revolves around established biotechnology workhorses”, figshare, Link to preprint
  127. Wenfa Ng, “Is TCA cycle activated under anaerobic conditions?”, figshare, Link to preprint
  128. Wenfa Ng, “Commercial kits have revolutionized and made accessible much of modern molecular biology”, figshare, Link to preprint
  129. Wenfa Ng, “MALDI-TOF mass spectrometry based microbial identification is sensitive to cultivation conditions of microbes that may yield extraneous protein peaks”, figshare, Link to preprint
  130. Wenfa Ng, “Expression level of housekeeping genes such as gyrase A could be used to normalize expression counts of genes in RNA-seq transcriptome”, figshare, Link to preprint
  131. Wenfa Ng, “Batteries as energy storage nexus connecting solar power with grid power for stable provision of electricity”, figshare, Link to preprint
  132. Wenfa Ng, “Ethanol utilization capability likely evolve later than ethanol production capability in the aldehyde-alcohol dehydrogenase, adhE of Escherichia coli”, figshare, Link to preprint
  133. Wenfa Ng, “Pluto does not belong to our Solar System as its centre of orbital rotation is not the Sun and it does not lie in the ecliptic plane of the Solar System”, figshare, Link to preprint
  134. Wenfa Ng, “Elevated engine noise at back of commuter bus”, figshare, Link to preprint
  135. Wenfa Ng, “Relative abundance of enzymes in a pathway offer clues to rate-limiting step of the pathway”, figshare, Link to preprint
  136. Wenfa Ng, “Analyzing the evolutionary fitness advantage of contact-dependent injection of toxic effectors by Type 6 secretion systems in bacteria”, figshare, Link to preprint
  137. Wenfa Ng, “Machine learning models for predicting enzymatic activity are specific only for the class of enzyme”, figshare, Link to preprint
  138. Wenfa Ng, “Mathematical modelling helps clarify the theoretical basis of physiochemical and biological phenomena”, figshare, Link to preprint
  139. Wenfa Ng, “Identifying central carbon metabolism intermediates that serve as decision matrix for entry into cell division”, figshare, Link to preprint
  140. Wenfa Ng, “Gut microbiome of wild animals may be rich in species that secrete digestive enzymes useful for biotechnology”, figshare, Link to preprint
  141. Wenfa Ng, “Evolutionary role of glycolysis may be to breakdown large molecule to respective C2, C3 and C5 building blocks for supporting biomass formation”, figshare, Link to preprint
  142. Wenfa Ng, “Anaerobic metabolism in gut environment may constraint the growth of pathogens”, figshare, Link to preprint
  143. Wenfa Ng, “Expression of formate dehydrogenase in Methanobrevibacter smithii may be induced by presence of formate”, figshare, Link to preprint
  144. Wenfa Ng, “Ribo-seq proteomics provides a complementary view of cellular proteome at a particular point in time”, figshare, Link to preprint
  145. Wenfa Ng, “Conjugation of biomolecules with green fluorescent protein may affect protein diffusional dynamics and subcellular localization”, figshare, Link to preprint
  146. Wenfa Ng, “Force fields provide a faster means for computational chemistry modelling compared to atomistic simulations”, figshare, Link to preprint
  147. Wenfa Ng, “Cross-talks in metabolic and signalling networks may prevent implementation of modular heterologous pathways in biology”, figshare, Link to preprint
  148. Wenfa Ng, “Possibility of horizontal transfer of Ars operon from arsenic tolerant microbe to Escherichia coli K-12 through groundwater consumption by humans”, figshare, Link to preprint
  149. Wenfa Ng, “Cofactor regeneration could only occur if the speed of the biotransformation reaction and cofactor regeneration reaction are similar”, figshare, Link to preprint
  150. Wenfa Ng, “Do we experimentally screen or computationally model a library of protein variants in directed evolution experiments?”, figshare, Link to preprint
  151. Wenfa Ng, “Absence of sequence homology of phage genes with those of other bacteriophage suggests that PBSX prophage in Bacillus subtilis 168 is extinct in the environment”, figshare, Link to preprint
  152. Wenfa Ng, “Bacteriophage genome expands with splicing in of host genes through transposase activity”, figshare, Link to preprint
  153. Wenfa Ng, “Prophage genes with no expression level in RNA-seq analysis are likely to be true phage genes”, figshare, Link to preprint
  154. Wenfa Ng, “Lysogeny may present a selective disadvantage for bacteriophages”, figshare, Link to preprint
  155. Wenfa Ng, “Bacteriophage integrase mediates the integration of the phage genome into the host genome during lysogeny”, figshare, Link to preprint
  156. Wenfa Ng, “Tight binding between ACE2 receptor and spike protein hints at the potential for developing ACE2 as therapeutic neutralizing antibody for SARS-CoV-2”, figshare, Link to preprint
  157. Wenfa Ng, “Possible presence of neutralizing antibodies for gut phages in human blood”, figshare, Link to preprint
  158. Wenfa Ng, “Cannibalistic pathways in Bacillus subtilis should be knockout to help protect cell population from lysis in biotechnology applications”, figshare, Link to preprint
  159. Wenfa Ng, “Characterizing ethanol-sensitive promoters and understanding the evolutionary forces acting on them in common biotechnology chassis”, figshare, Link to preprint
  160. Wenfa Ng, “Developing an ethanol responsive regulon in Escherichia coli for biotechnology applications”, figshare, Link to preprint
  161. Wenfa Ng, “Lysogeny may not have evolved as a mechanism for creating a reservoir of phages in bacterial cells”, figshare, Link to preprint
  162. Wenfa Ng, “High expression level of transposase does not translate to fast evolution rate of a microbial species”, figshare, Link to preprint
  163. Wenfa Ng, “Lack of homologous genes in other bacteriophages meant that many of the prophages in Escherichia coli K-12 are extinct in the environment”, figshare, Link to preprint
  164. Wenfa Ng, “Genes in extinct prophages typically lack sequence homology to genes in other bacteriophages”, figshare, Link to preprint
  165. Wenfa Ng, “Unequal access of bacteriophages to microorganisms across different domains of life”, figshare, Link to preprint
  166. Wenfa Ng, “Large and diverse genetic repertoire suggests that bacteriophages might have evolved as an independent life-form prior to parasitic mode of life we see today”, figshare, Link to preprint
  167. Wenfa Ng, “Viruses that cross species barrier should have low transmission rates in the new population”, figshare, Link to preprint
  168. Wenfa Ng, “Some ribosomal proteins are not omnipresent in bacterial species thereby hinting at their nonessentiality in ribosome biogenesis”, figshare, Link to preprint
  169. Wenfa Ng, “Bacterial genomes may be living chromometer of prophages that have become extinct in the environment”, figshare, Link to preprint
  170. Wenfa Ng, “Combination of BLAST and phylogenetic tree analysis provide a more rounded view of molecular phylogeny”, figshare, Link to preprint
  171. Wenfa Ng, “Lack of alignment in promoter sequence of human genes suggest paucity of long-range conserved motifs in human promoter sequence”, figshare, Link to preprint
  172. Wenfa Ng, “Predictions on regulatory logic of glycerol metabolism in Escherichia coli”, figshare, Link to preprint
  173. Wenfa Ng, “Glucose replete conditions repress adhE expression to direct more flux to tricarboxylic acid cycle”, figshare, Link to preprint
  174. Wenfa Ng, “Formate assimilation pathway likely first evolved in microbes but was lost due to dominance of glucose as carbon source as Earth’s environment evolves”, figshare, Link to preprint
  175. Wenfa Ng, “Machine learning models are derived based on correlations rather than mechanistic insights”, figshare, Link to preprint
  176. Wenfa Ng, “Optimal proportion of renewable energy in energy mix for maximising energy resilience and reducing carbon emissions”, figshare, Link to preprint
  177. Wenfa Ng, “Theoretical research suggests hypotheses for experimental science”, figshare, Link to preprint
  178. Wenfa Ng, “Perturbation yields new insights about a system”, figshare, Link to preprint
  179. Wenfa Ng, “Pyruvate as potential carbon entry point to central carbon metabolism from ethylene glycol”, figshare, Link to preprint
  180. Wenfa Ng, “Biomolecular condensates lacking functions should not be called membraneless organelles”, figshare, Link to preprint
  181. Wenfa Ng, “Difficulty lies in estimating the evolutionary age of specific microbial species”, figshare, Link to preprint
  182. Wenfa Ng, “Proteins possess many more conformation states than those available for interrogation by protein X-ray crystallography”, figshare, Link to preprint
  183. Wenfa Ng, “Some ribosomal proteins may be nonessential and evolve later in the biogenesis of ribosome”, figshare, Link to preprint
  184. Wenfa Ng, “Metabolic side branches to glycolysis are likely to have evolved later compared to the main metabolic highway”, figshare, Link to preprint
  185. Wenfa Ng, “Need for cellular RNA polymerase for transcribing viral genes present a conundrum for provenance of viral genome”, figshare, Link to preprint
  186. Wenfa Ng, “Small proteins serve an evolutionary important role in facilitating intercellular communication, sensory and effector function”, figshare, Link to preprint
  187. Wenfa Ng, “Quality of RNA-seq sequence library depends critically on use of high-fidelity reverse transcriptase with proof-reading ability”, figshare, Link to preprint
  188. Wenfa Ng, “Genome wide tuning of promoter strength for delivering optimized enzyme levels meant evolution likely solved a system optimization problem in promoter engineering”, figshare, Link to preprint
  189. Wenfa Ng, “Bacillus subtilis is a compelling model organism for bacterial cell differentiation programmes and multicellularity”, figshare, Link to preprint
  190. Wenfa Ng, “High transcriptional activity across many genes in Metallosphaera sedula DSM 5348T at growth pH 1.5 and 2”, figshare, Link to preprint
  191. Wenfa Ng, “Phosphorylation serves as memory counter of different conformational and functional states of protein”, figshare, Link to preprint
  192. Wenfa Ng, “Lack of access to full-text of journal articles behind paywall presents significant challenges to citizen science”, figshare, Link to preprint
  193. Wenfa Ng, “Mitochondria ribosomal protein genes in many eukaryotic species have been transferred from the mitochondria to nuclear genome”, figshare, Link to preprint
  194. Wenfa Ng, “Most open reading frames in bacteriophage genome are of unknown function”, figshare, Link to preprint
  195. Wenfa Ng, “Theoretical framework of de novo protein structure prediction exists but require support from more sophisticated computing paradigm”, figshare, Link to preprint
  196. Wenfa Ng, “Exopolysaccharide biosynthesis protein may confer fitness advantages to bacteriophage phi3T of Bacillus subtilis”, figshare, Link to preprint
  197. Wenfa Ng, “RNA-seq transcriptome data could help identify strong promoters useful for biotechnology”, figshare, Link to preprint
  198. Wenfa Ng, “Machine-learning based protein structure prediction methods are only specific for particular class of proteins”, figshare, Link to preprint
  199. Wenfa Ng, “MALDI-TOF imaging mass spectrometry detection of proteins after separation of whole cell lysate by thin polyacrylamide gel electrophoresis”, figshare, Link to preprint
  200. Wenfa Ng, “MALDI-TOF imaging mass spectrometry detection of proteins after separation of whole cell lysate by thin polyacrylamide gel electrophoresis”, figshare, Link to preprint
  201. Wenfa Ng, “Problems with large number of variables need a large dataset for efficient training of machine learning algorithms”, figshare, Link to preprint
  202. Wenfa Ng, “Genes in pathogenicity island 1 of Salmonella typhimurium are highly expressed during planktonic growth in rich LB medium”, figshare, Link to preprint
  203. Wenfa Ng, “Coupling of natural products biosynthetic gene cluster with weak promoter help titrate production of antimicrobial compounds”, figshare, Link to preprint
  204. Wenfa Ng, “Interrogating the cell surface proteome of tumour cells in search for antigens useful in cancer recognition and targeting”, figshare, Link to preprint
  205. Wenfa Ng, “Feasibility of titrating the expression of genes in a pathway based on concentration of toxic intermediates”, figshare, Link to preprint
  206. Wenfa Ng, “Molecular docking experiments could offer glimpse into why a particular enzyme has low activity with respect to a given substrate”, figshare, Link to preprint
  207. Wenfa Ng, “Native photorespiration reaction in plants may have been optimized through evolutionary natural selection pressure”, figshare, Link to preprint
  208. Wenfa Ng, “Global regulatory factor for transcription may be identifiable by their multitude of binding sites for different inducers, substrates and DNA sequence”, figshare, Link to preprint
  209. Wenfa Ng, “Determining the substrate scope of an enzyme through molecular docking experiments”, figshare, Link to preprint
  210. Wenfa Ng, “Obtaining enzyme-substrate complex crystal is a difficult step in X-ray crystallography”, figshare, Link to preprint
  211. Wenfa Ng, “Tedious biochemical assays remain the rate-limiting factor as we explore the functions of proteins”, figshare, Link to preprint
  212. Wenfa Ng, “Integration of sequence and structural homology in search for conservation principles and similarity examples in biology”, figshare, Link to preprint
  213. Wenfa Ng, “Reducing diffusional distance of intermediates by assembling enzymes of a common pathway on a scaffold”, figshare, Link to preprint
  214. Wenfa Ng, “Expanding physicians’ view of microbes beyond the traditional biased view of microbes as pathogens”, figshare, Link to preprint
  215. Wenfa Ng, “Homology modelling could provide approximate structures of proteins encoded by the SARS-CoV-2 genome”, figshare, Link to preprint
  216. Wenfa Ng, “Assessing the proof-reading ability of viral polymerases to determine their pathogenic potential”, figshare, Link to preprint
  217. Wenfa Ng, “Importance of assessing the replicative fidelity of viral polymerases in determining virus pathogenic potential”, figshare, Link to preprint
  218. Wenfa Ng, “Codon utilisation pattern is unique to each species and represents a species boundary”, figshare, Link to preprint
  219. Wenfa Ng, “Cell culture remains the most accessible technique for unbiased profiling of microbes in blood and tissue samples”, figshare, Link to preprint
  220. Wenfa Ng, “Homology modelling could provide approximate structures of proteins encoded by the SARS-CoV-2 genome”, figshare, Link to preprint
  221. Wenfa Ng, “RNA-seq high cost structure precludes its use as clinical diagnostics for routine microbial identification”, figshare, Link to preprint
  222. Wenfa Ng, “Ability to help assort genes in different configurations help blunt TA ligase survive evolutionary selection”, figshare, Link to preprint
  223. Wenfa Ng, “Flat tissue culture flask mimics the mechanobiology environment of human tissue in providing a surface for cell attachment and growth”, figshare, Link to preprint
  224. Wenfa Ng, “Significant difficulties exist in obtaining complete understanding of microbial species represented in a metagenome”, figshare, Link to preprint
  225. Wenfa Ng, “Overview of methods for detecting SARS-CoV-2 virus”, figshare, Link to preprint
  226. Wenfa Ng, “Overview of how technology has aided the detection of SARS-CoV-2”, figshare, Link to preprint
  227. Wenfa Ng, “Mutational rate of viruses and microbial pathogen may be intimately linked to proof-reading ability of its polymerase”, figshare, Link to preprint
  228. Wenfa Ng, “BLAST analysis of urease gene confirms its specificity for identifying Helicobacter pylori in qPCR assays”, figshare, Link to preprint
  229. Wenfa Ng, “Alternative design of a bacterial transformation chip”, figshare, Link to presentation
  230. Wenfa Ng, “Genes evolve at different rates in a species in a process dependent on evolutionary pressure”, figshare, Link to preprint
  231. Wenfa Ng, “No endogenized phage genes in Mesoplasma florum”, figshare, Link to preprint
  232. Wenfa Ng, “Assessing the feasibility of culturing Escherichia coli cells in mammalian tissue culture flask in a curiosity driven project”, SSRN, Link to preprint
  233. Wenfa Ng, “Development of a MATLAB error-prone PCR simulator for random mutation of target gene”, SSRN, Link to preprint
  234. Wenfa Ng, “MATLAB software for obtaining promoter sequence of target gene from an annotated genome of a bacterial species”, SSRN, Link to preprint
  235. Wenfa Ng, “Widespread Cell Lysis Amidst Large Number of Dead Cells in 121-day old Escherichia coli Liquid Culture”, SSRN, Link to preprint
  236. Wenfa Ng, “Molecular and cellular speciation mechanisms complement speciation mechanisms based on morphological and phenotypic characteristics”, SSRN, Link to preprint
  237. Wenfa Ng, “Ring species are natural experiments for understanding the relative contribution of genetic replication machinery and outward appearance on speciation”, SSRN, Link to preprint
  238. Wenfa Ng, “Gene flow in humans may potentiate language evolution”, SSRN, Link to preprint
  239. Wenfa Ng, “Answering whether evolutionary outcomes are unique through parallel adaptive evolution experiments”, SSRN, Link to preprint
  240. Wenfa Ng, “Defining a synthetic microbial consortium for activated sludge municipal wastewater treatment”, SSRN, Link to preprint
  241. Wenfa Ng, “Life adapts to glucose within a narrow range that optimizes metabolism and growth”, figshare, Link to preprint
  242. Wenfa Ng, “Multiplex MALDI-TOF-MS microbial identification could aid the identification of multiple microbial contaminants in food through proteome database search approach”, figshare, Link to preprint
  243. Wenfa Ng, “Modern molecular dynamics simulation aids in discovering more conformational states and putative functions of a protein with a solved structure”, figshare, Link to preprint
  244. Wenfa Ng, “Molecular docking experiments with putative substrate of enzyme could confirm purported enzyme active site”, figshare, Link to preprint
  245. Wenfa Ng, “Enzyme with broad substrate scope typically have large access tunnel and bind to the reaction centre of the substrate”, figshare, Link to preprint
  246. Wenfa Ng, “Evolving enzymes with expanded substrate range for wastewater treatment applications”, figshare, Link to preprint
  247. Wenfa Ng, “Gene expression analysis helps uncover potential stress response of cells engineered for utilizing unconventional substrate”, figshare, Link to preprint
  248. Wenfa Ng, “Enzyme variant with least divergence from a conserved sequence for the enzyme should have reasonable amount of activity and should form the basis for machine learning modelling”, figshare, Link to preprint
  249. Wenfa Ng, “Genes evolve at different rates in a species in a process dependent on evolutionary pressure”, figshare, Link to preprint
  250. Wenfa Ng, “Inability to design precise enzyme movement may explain poor performance of de novo designed enzymes”, figshare, Link to preprint
  251. Wenfa Ng, “Assessing if protein variants arising from alternative splicing follow a stochastic distribution”, figshare, Link to preprint
  252. Wenfa Ng, “Possibility of using CRISPR-Cas9 for inactivating genes controlling cell differentiation pathways in Bacillus subtilis”, figshare, Link to preprint
  253. Wenfa Ng, “Low affinity binding between transcriptional repressor and inducer may result in poor control of gene transcription and higher gene expression noise”, figshare, Link to preprint
  254. Wenfa Ng, “Engagement of cannibalistic behaviour during stationary phase hints that Bacillus subtilis could not enter low energy mode typified in persister state”, figshare, Link to preprint
  255. Wenfa Ng, “Possibility exists in using co-precipitation and LC-MS to elucidate the binding partner of a nanoparticle conjugated drug for understanding potential side effects profile”, figshare, Link to preprint
  256. Wenfa Ng, “Phylogenetic chronometer biomolecules chronicles both long and short evolutionary distance accurately”, figshare, Link to preprint
  257. Wenfa Ng, “Probing expression of specific pathway genes with targeted RNA sequencing”, figshare, Link to preprint
  258. Wenfa Ng, “Phylogenetic signals of 18S rRNA are encoded in sequence region not in contact with other biomolecules and is without function”, figshare, Link to preprint
  259. Wenfa Ng, “Profiling the genetic repertoire of the gut microbiome for understanding causes of metabolic syndrome in humans”, figshare, Link to preprint
  260. Wenfa Ng, “Presence of large number of transposase and transposase helper genes in a bacterial species meant that it is not suited as biotechnology chassis”, figshare, Link to preprint
  261. Wenfa Ng, “Relative high activity and moderate relative abundance of metabolic enzyme in central carbon metabolism suggests evolutionary hand in enzyme activity tuning”, figshare, Link to preprint
  262. Wenfa Ng, “Random mutagenesis cum high throughput screening may be better able to survey a fitness landscape compared to long term adaptive evolution experiment”, figshare, Link to preprint
  263. Wenfa Ng, “Requirement for large population in a hostile habitat meant that evolution of Helicobacter pylori in the human stomach is retarded”, figshare, Link to preprint
  264. Wenfa Ng, “Relatively poor understanding of microbes’ ecological roles awaits system biology elucidation”, figshare, Link to preprint
  265. Wenfa Ng, “Quality of homology structural model differs but quality of model is better for proteins with many solved experimental structures”, figshare, Link to preprint
  266. Wenfa Ng, “Reversible enzymes have common domains for substrate and product”, figshare, Link to preprint
  267. Wenfa Ng, “Ribosomes with high activity and translation efficiency affords microbes to quickly adapt to changes in their immediate environmental and nutritional conditions”, figshare, Link to preprint
  268. Wenfa Ng, “Selecting a high activity enzyme through experiment may bring forth a bigger process improvement compared to extensive protein engineering of a mid-range candidate”, figshare, Link to preprint
  269. Wenfa Ng, “Some bacterial species activate multitude of gene expression programmes evenly while others use a tiered approach”, figshare, Link to preprint
  270. Wenfa Ng, “Understanding and engineering the secretome of biotechnology workhorse may simplify and reduce the cost of downstream product purification and recovery”, figshare, Link to preprint
  271. Wenfa Ng, “Understanding differential gene expression of cells in the non-growing state may inform enzyme targets useful for cofactor regeneration”, figshare, Link to preprint
  272. Wenfa Ng, “Use of RNA-seq to identify causes of slow expression kinetics of microbes to new nutrient substrate”, figshare, Link to preprint
  273. Wenfa Ng, “Use of structure prediction and molecular docking tools to assess the binding affinity of particular drugs on mutated proteins”, figshare, Link to preprint
  274. Wenfa Ng, “Using the tools of metabolomics to profile the ensemble of metabolites in each human organ”, figshare, Link to preprint
  275. Wenfa Ng, “Utilising pathway engineering in shaping a synthetic microbial consortium for municipal wastewater treatment”, figshare, Link to preprint
  276. Wenfa Ng, “Bacterial DNA not found in Neanderthal whole genome shotgun sequencing reads in a preliminary study”, figshare, Link to preprint
  277. Wenfa Ng, “Horizontal gene transfer may be more prevalent in closely related bacterial and microbial species”, figshare, Link to preprint

Dataset

  1. Wenfa Ng, “Effect of temperature on growth of Pseudomonas protegens Pf-5 and Pseudomonas aeruginosa PRD-10 in LB Lennox medium”, PeerJ Preprints, Link to preprint
  2. Wenfa Ng, “Effect of glucose and ammonium chloride supplementation and phosphate buffer on Escherichia coli DH5α growth in LB Lennox medium”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng, “Growth of Pseudomonas protegens Pf-5 in M9 minimal salts medium”, PeerJ Preprints, Link to preprint
  4. Wenfa Ng, “Database of all genes in Escherichia coli K-12 MG1655 which are not susceptible to restriction enzyme digestion by NdeI and BamHI”, figshare, Link to dataset
  5. Wenfa Ng, “Database of primers of genes in Escherichia coli K-12 without recognition sequence of NdeI and BamHI”, figshare, Link to dataset
  6. Wenfa Ng, “Proteome database of Escherichia coli K-12”, figshare, Link to dataset
  7. Wenfa Ng, “Gene database of Saccharomyces cerevisiae S288C”, figshare, Link to dataset
  8. Wenfa Ng, “CRISPR-Cas9 spacer sequence for targeting ldhA gene in Escherichia coli K-12 MG1655”, figshare, Link to dataset
  9. Wenfa Ng, “Gene database for Bacillus subtilis 168”, figshare, Link to dataset
  10. Wenfa Ng, “Transcriptome of Escherichia coli K-12 (MG1655) at stationary phase in minimal medium”, figshare, Link to dataset
  11. Wenfa Ng, “Database of ribosome binding site of all genes in Escherichia coli K-12 MG1655”, figshare, Link to dataset
  12. Wenfa Ng, “Spacer sequence for targeting gyrA gene in Bacillus subtilis strain 168 using CRISPR-Cas9”, figshare, Link to dataset
  13. Wenfa Ng, “Transcriptome dataset of Bacillus subtilis 168 cultivated in LB medium”, figshare, Link to dataset
  14. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas aeruginosa PAO1 in Brain Heart Infusion broth”, figshare, Link to dataset
  15. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas putida KT2440 fed with glucose as sole carbon source”, figshare, Link to dataset
  16. Wenfa Ng, “RNA-seq transcriptome of Staphylococcus aureus at stationary phase in Tryptic Soy Broth”, figshare, Link to dataset
  17. Wenfa Ng, “RNA-seq transcriptome of Staphylococcus aureus MRSA at mid-exponential phase”, figshare, Link to dataset
  18. Wenfa Ng, “Determining the amino acid composition of every protein in Escherichia coli K-12”, figshare, Link to dataset
  19. Wenfa Ng, “Codon usage pattern of all genes in Escherichia coli K-12”, figshare, Link to dataset
  20. Wenfa Ng, “Elemental compositions of deionized water and tap water”, figshare, Link to preprint
  21. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas putida KT2440 subsisting on glycerol as sole carbon source”, figshare, Link to dataset
  22. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas putida KT2440 subsisting on fructose as sole carbon source”, figshare, Link to dataset
  23. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas putida KT2440 subsisting on succinate as sole carbon source”, figshare, Link to dataset
  24. Wenfa Ng, “Effect of yeast extract supplementation on growth of Pseudomonas aeruginosa PRD-10 in M9 medium”, figshare, Link to preprint
  25. Wenfa Ng, “Average codon usage pattern of Escherichia coli K-12”, figshare, Link to dataset
  26. Wenfa Ng, “Attempted fractionation of LB Lennox medium via reversed phase high performance liquid chromatography”, bioRxiv, Link to preprint
  27. Wenfa Ng, “Understanding the compositional changes in LB Lennox medium during growth of Escherichia coli DH5α in a 1L bioreactor”, bioRxiv, Link to preprint
  28. Wenfa Ng, “Growth of Bacillus subtilis NRS-762 (ATCC 8473) in different growth medium under different temperatures”, figshare, Link to dataset
  29. Wenfa Ng, “RNA-seq transcriptome of Pseudomonas syringae DC3000 in liquid minimal medium”, figshare, Link to dataset
  30. Wenfa Ng, “Growth of Chromobacterium violaceum on R2A and Tryptic Soy Agar”, figshare, Link to dataset
  31. Wenfa Ng, “Dataset on assessment of thermodynamic feasibility of a variety of ketone to alcohol transformations suited for biocatalysis”, figshare, Link to preprint
  32. Wenfa Ng, “Dataset of scanning electron microscopy images of freeze-dried Escherichia coli DH5α cells”, figshare, Link to preprint
  33. Wenfa Ng, “Dataset of elemental composition of LB Lennox (buffered) medium with and without glucose supplementation”, figshare, Link to preprint
  34. Wenfa Ng, “Dataset on correlation plots of expected and measured concentrations from ICP-MS for a range of elements in ICP-MS multi-element calibration standard”, figshare, Link to preprint
  35. Wenfa Ng, “Growth of Bacillus subtilis NRS-762, Pseudomonas aeruginosa PRD-10, Pseudomonas protegens Pf-5, Escherichia coli DH5α, and Chromobacterium violaceum on LB Lennox agar”, figshare, Link to preprint
  36. Wenfa Ng, “Dataset describing homology structures of ribosomal protein L36 of different microbial species”, figshare, Link to dataset
  37. Wenfa Ng, “Viable cell count of Escherichia coli DH5α in LB Lennox medium supplemented with 2 g/L glucose”, figshare, Link to preprint
  38. Wenfa Ng, “Dataset of frequency of occurrence of words in title of journal articles of different fields in biology”, figshare, Link to dataset
  39. Wenfa Ng, “Dataset of phage genes in Escherichia coli K-12”, figshare, Link to dataset
  40. Wenfa Ng, “Dataset of phage genes in Bacillus subtilis 168”, figshare, Link to dataset
  41. Wenfa Ng, “Collection of putative promoter sequence of genes on different chromosomes of the human genome”, figshare, Link to dataset
  42. Wenfa Ng, “Gene database of genes on different human chromosomes”, figshare, Link to dataset
  43. Wenfa Ng, “Ribosomal protein library of different microbial species”, figshare, Link to preprint
  44. Wenfa Ng, “Significant number of small proteins with less than 50 amino acids remain uncharacterized in different microbial species”, figshare, Link to dataset
  45. Wenfa Ng, “Comprehensive library of small proteins of different microbial species”, figshare, Link to dataset
  46. Wenfa Ng, “Comprehensive library of ribosomal proteins of different microbial species”, figshare, Link to dataset
  47. Wenfa Ng, “Analysis of the types of samples that could be handled by RT-qPCR COVID-19 test in Singapore”, figshare, Link to dataset
  48. Wenfa Ng, “Dataset of COVID-19 diagnostic tests that have been developed around the world”, figshare, Link to dataset
  49. Wenfa Ng, “Collection of structures of proteins encoded by the SARS-CoV-2 genome obtained through homology modelling”, Open Science Framework, Link to dataset
  50. Wenfa Ng, “Dataset annotating different features of human cell lines in biological and biomedical research”, figshare, Link to dataset
  51. Wenfa Ng, “Dataset of partial gene sequence of new-to-nature microbial species identified in a normal mouse gut microbiome”, figshare, Link to dataset

Tools

  1. Wenfa Ng, “Do it yourself styrofoam microtube holder for better organization of microtubes on ice”, figshare, Link to preprint
  2. Wenfa Ng, “Molecular weight calculator for organic compounds in biotechnology”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng, “MATLAB software for extracting protein name and sequence information from FASTA formatted proteome file”, PeerJ Preprints, Link to preprint
  4. Wenfa Ng, “MATLAB software for automated calculation of concentration of different compounds using extracted peak area from HPLC data file in pdf format”, PeerJ Preprints, Link to preprint
  5. Wenfa Ng, “MATLAB software that calculates molecular weight of protein from gene sequence”, figshare, Link to preprint
  6. Wenfa Ng, “Python software for calculating the area under a curve”, figshare, Link to preprint
  7. Wenfa Ng, “Python software for determining the time profile of reactant concentration for a first order reaction”, figshare, Link to preprint
  8. Wenfa Ng, “MATLAB software for constructing a gene database from annotated genome sequence of a prokaryotic species”, figshare, Link to preprint
  9. Wenfa Ng, “MATLAB software for building gene catalogue of eukaryotic organism and microbiome”, figshare, Link to preprint
  10. Wenfa Ng, “Protocol for the transformation of Bacillus subtilis through inducing natural competence”, figshare, Link to preprint
  11. Wenfa Ng, “Automated screening of DNA sequence of gene for recognition sequence of specific restriction enzymes”, figshare, Link to preprint
  12. Wenfa Ng, “MATLAB software suite that process raw RNA-seq transcriptome dataset on a personal computer”, figshare, Link to preprint
  13. Wenfa Ng, “Diagnostic software for RNA-seq and Ribo-seq data analysis for assessing quality of sequenced reads”, figshare, Link to preprint
  14. Wenfa Ng, “Protocol for anaerobic two phase resting cell biotransformation experiment”, figshare, Link to preprint
  15. Wenfa Ng, “MATLAB software for obtaining promoter sequence of target gene in bacterial species”, figshare, Link to preprint
  16. Wenfa Ng, “MATLAB software for identification of consensus sequence after multiple sequence alignment”, figshare, Link to preprint

Commentary

  1.  Wenfa Ng, “Combining different experiment and instrument approaches to  reconstruct how a protein moves and function”, figshare, Link to peer review, Link to post on Publons
  2. Wenfa Ng, “Post antibiotic treatment expansion of pathogenic gut microbes induced by availability of oxidised sugars”, figshare, Link to peer review, Link to post on Publons
  3. Wenfa Ng, “Ethanol pretreatment and bile acid germinants help recover significant number of spore forming gut bacteria previously unculturable”, figshare, Link to peer review, Link to post on Publons
  4. Wenfa Ng, “Transmissible cancer in marine bivalves: Implications for mode of cancer transmission”, PeerJ Preprints, Link to peer review, Link to post on Publons
  5. Wenfa Ng, “Understanding evolutionary dynamics of phosphorylation with fungal species as model system”, PeerJ Preprints, Link to peer review, Link to post on Publons
  6. Wenfa Ng, “Light-based patterning of bacterial cells through induction of biofilm formation processes”, PeerJ Preprints, Link to peer review, Link to post on Publons
  7. Wenfa Ng, “Scientific possibilities in associating microbiome to specific diseases and its bioinformatic and experimental challenges”, PeerJ Preprints, Link to peer review, Link to post on Publons
  8. Wenfa Ng, “Review of genetics-free high-throughput approach for activating production of cryptic microbial secondary metabolites”, figshare, Link to preprint
  9. Wenfa Ng, “Review of using diacetic functionalized polymers in solubilizing enzymes in organic solvents”, figshare, Link to preprint
  10. Wenfa Ng, “Spotlight on using constructed ecosystem for understanding the impacts of climate change at the mesocosm level”, figshare, Link to preprint
  11. Wenfa Ng, “Constraints of cell growth in narrow microchannels”, figshare, Link to preprint
  12. Wenfa Ng,”Highlight on software that predicts overexpression strategies for overproduction of metabolites”, figshare, Link to preprint
  13. Wenfa Ng, “Contemporary perspective on a foundational paper in role of structural maintenance of chromosome complex in mediating DNA damage response checkpoint”, figshare, Link to preprint
  14. Wenfa Ng, “Inertial migration of neutrally buoyant particles in superhydrophobic channels: An evaluation”, figshare, Link to preprint

Analysis

  1. Wenfa Ng, “Should groundwater be used for drinking purposes?” fighare, Link to analysis
  2. Wenfa Ng, “Overabundance of renewable energy lead to removal of market distorting feed-in tariffs”, figshare, Link to analysis
  3. Wenfa Ng, “Li-Fi and its challenges to practical use”, figshare, Link to analysis
  4. Wenfa Ng, “Energy use in water purification as criterion for selecting drinking water treatment technologies”, PeerJ Preprints, Link to analysis
  5. Wenfa Ng, “Fuel efficiency standards’ roles in reducing greenhouse gas emissions and air pollutants”, PeerJ Preprints, Link to analysis
  6. Wenfa Ng, “Setting a price for carbon for implementing a carbon tax or a cap and trade system for controlling carbon dioxide emissions”, PeerJ Preprints, Link to analysis
  7. Wenfa Ng, “Research institutes complement universities by providing targeted focus on particular research topics”, figshare, Link to analysis
  8. Wenfa Ng, “Design considerations in predicting energy needs of spacecraft”, figshare, Link to analysis

Synopsis

  1. Wenfa Ng, “Identifying microbes from environment water samples in a discovery-based learning module”, PeerJ Preprints, Link to preprint
  2. Wenfa Ng, “Patterning bacteria on agar for understanding distance mediated cell-cell signaling and metabolic exchanges”, PeerJ Preprints, Link to preprint
  3. Wenfa Ng, “Inability to completely remove trace contaminants from drinking water by adsorption”, PeerJ Preprints, Link to preprint

Economics research

  1. Wenfa Ng, “Are advanced economies increasingly reliant on speculative activities in financial markets and fiscal stimulus to nudge anemic growth upwards?”, Social Science Research Network (SSRN), Link to preprint
  2. Wenfa Ng, “Economic disparities portend a four speed proposed ASEAN monetary union with implications for regional financial and economic stability”, Social Science Research Network (SSRN), Link to preprint
  3. Wenfa Ng, “Quasi independence of most central banks: Implications for monetary policy in advanced countries and beyond”, Social Science Research Network (SSRN), Link to preprint
  4. Wenfa Ng, “Value and disvalue of a strong currency”, Social Science Research Network (SSRN), Link to preprint
  5. Wenfa Ng, “Effect of different funding and operating models of public transport on cost and efficiency of bus and rail services”, Social Science Research Network (SSRN), Link to preprint
  6. Wenfa Ng, “Setting a value for a currency: Interest rate, bank reserve ratio or pegging to a basket of currencies of major trading partners”, Social Science Research Network (SSRN), Link to preprint
  7. Wenfa Ng, “Price of currency based on terms of trade is less vulnerable to manipulation but is open to contribution from inflationary pressure”, figshare, Link to preprint
  8. Wenfa Ng, “Terms of trade calculation as guide for currency value within which free market mechanisms finetunes a currency price”, figshare, Link to preprint
  9. Wenfa Ng, “Concept of liquidity should be broadened to include transaction time and rate of variation of value during use of said liquidity”, figshare, Link to preprint
  10. Wenfa Ng, “State ownership of public transportation assets”, figshare, Link to preprint
  11. Wenfa Ng, “Choice of subsidy or one-time low interest loans depends on economic efficiency, possible modulation of social behaviour or creation of new industries”, figshare, Link to preprint
  12. Wenfa Ng, “Solar power generation should ideally be integrated with conventional power company to create a reliable power network”, figshare, Link to preprint
  13. Wenfa Ng, “Reserve currency status underpins and underwrites the issuing of government bonds in advanced economies”, figshare, Link to preprint
  14. Wenfa Ng, “Inelastic nature of labour market needs a progressive wage structure to equilibrate skills and salary compensation”, figshare, Link to preprint
  15. Wenfa Ng, “Proper valuation of a currency keeps a currency’s price in sync with economic fundamentals and prevents asset bubbles and inflation”, figshare, Link to preprint
  16. Wenfa Ng, “The real economics of food and groceries delivery companies lies in valuation of time”, figshare, Link to preprint
  17. Wenfa Ng, “Logistical challenges for land freight into inland cities far from the coast”, figshare, Link to preprint
  18. Wenfa Ng, “Should central bank value a currency for increasing purchasing power or improve jobs creation?”, SSRN, Link to preprint
  19. Wenfa Ng, “Intermittency in supply highlights an inherent need for insurance coverage for commercial users of renewable power”, SSRN, Link to preprint
  20. Wenfa Ng, “Insurance as a form of social safety net”, SSRN, Link to preprint
  21. Wenfa Ng, “Full transparency between buyer and seller and perfect transmission of information are key to realising a perfect competition market mechanism”, SSRN, Link to preprint
  22. Wenfa Ng, “Does initial size of a company with network effect influence its survival?”, SSRN, Link to preprint
  23. Wenfa Ng, “Wide diversity of products and constrained storage space place severe logistical challenges on many retail stores”, SSRN, Link to preprint
  24. Wenfa Ng, “Identifying transport demand nexus helps guide the planning of intra-city rail and bus routes”, SSRN, Link to preprint
  25. Wenfa Ng, “Necessity to subsidize electricity bill for defining telecommunication network of small players in the telecommunication market”, SSRN, Link to preprint
  26. Wenfa Ng, “Large conglomerates tend to emerge in perfect competitive and regulation light economic environment”, SSRN, Link to preprint
  27. Wenfa Ng, “Economic systems with constrained supply potential tilt towards importation for alleviating demand shock”, SSRN, Link to preprint
  28. Wenfa Ng, “Demand forecasting for rail and bus services in a city”, SSRN, Link to preprint
  29. Wenfa Ng, “Connecting suburban towns to city centre through express buses increases inefficiency and raises route profitability issues”, SSRN, Link to preprint
  30. Wenfa Ng, “Restructuring of value and supply chain in world’s manufacturing would likely lead to reduced trade and container volume around the world”, SSRN, Link to preprint
  31. Wenfa Ng, “How to position empty containers along major trade routes?”, SSRN, Link to preprint
  32. Wenfa Ng, “Encouraging competition to reduce prices: An externality to auction theory for price discovery”, SSRN, Link to preprint
  33. Wenfa Ng, “Market distorting effects of targeted subsidies”, SSRN, Link to preprint
  34. Wenfa Ng, “Hidden externalities in the power dynamics between food vendors and food delivery service”, SSRN, Link to preprint
  35. Wenfa Ng, “Government subsidies or free market mechanism in funding science content of newspapers”, SSRN, Link to preprint
  36. Wenfa Ng, “User demand for 4G services shaped by public education into the advantages of high-speed mobile data: Is a want (video streaming) turned into a need by advertising?”, SSRN, Link to preprint
  37. Wenfa Ng, “Balancing demand with socioeconomic function: Optimization of bus routes in Singapore”, SSRN, Link to preprint
  38. Wenfa Ng, “Geographic organization of a city may predetermine the funding models for public transportation in search for maximizing utility for all stakeholders”, SSRN, Link to preprint
  39. Wenfa Ng, “Proprietary weaving technology and fabric material meant that clothing companies are monopolistic in their individual market segment”, SSRN, Link to preprint
  40. Wenfa Ng, “Monopolistic tendency in electric car charging market portends a regulatory model that involves periodic tender of contract for provision of electricity”, SSRN, Link to preprint
  41. Wenfa Ng, “Network effects determine the growth potential of taxi companies”, SSRN, Link to preprint
  42. Wenfa Ng, “Effect of different funding and operating models of public transport on cost and efficiency of bus and rail services”, SSRN, Link to preprint
  43. Wenfa Ng, “Limits in city space constraints car population growth: Implications for positive rate of car growth in size-limited cities”, SSRN, Link to preprint
  44. Wenfa Ng, “Theoretical reasoning identifies multi-product segment in a perfectly competitive market”, SSRN, Link to preprint
  45. Wenfa Ng, “Proposed methodology for estimating the value of brands and products”, SSRN, Link to preprint
  46. Wenfa Ng, “What factors potentiate collaboration between divergent classes of businesses?”, SSRN, Link to preprint
  47. Wenfa Ng, “Asset bubbles are typically followed by financial crisis if the underlying economic externality is ameliorated suddenly”, SSRN, Link to preprint
  48. Wenfa Ng, “Rising Inflation after Economic Re-Opening from a Pandemic Could Be Due to Lack of Spare Production Capacity at Home”, SSRN, Link to preprint
  49. Wenfa Ng, “Proposal of a salary structure based on educational qualifications and work experience”, SSRN, Link to preprint
  50. Wenfa Ng, “Modern labour market is highly segmented and its microeconomic fundamentals are not perfectly competitive”, SSRN, Link to preprint
  51. Wenfa Ng, “Prevalence of auction theory for allocating resources in different markets will lead to higher Gini coefficient”, SSRN, Link to preprint
  52. Wenfa Ng, “Factoring the motivations of managerial personnel in modelling a firm”, SSRN, Link to preprint
  53. Wenfa Ng, “Valuing the quality of political leadership in understanding the economic growth of a nation”, SSRN, Link to preprint
  54. Wenfa Ng, “Perfect competition-based allocation may lead to inefficient resource allocation that may magnify into severe economic disequilibrium at the national level”, figshare, Link to preprint
  55. Wenfa Ng, “Efficient allocation of resources across different sectors of economy remains a significant challenge in contemporary macroeconomics”, SSRN, Link to preprint
  56. Wenfa Ng, “Dividend yields as lagging indicators of economic performance of specific market sectors”, SSRN, Link to preprint
  57. Wenfa Ng, “Proliferation of bipartite free trade agreement distorts the world’s food commodity markets”, SSRN, Link to preprint
  58. Wenfa Ng, “High proportion of social spending in government budget may signify income stagnation arising from hollowing out of mid-tier manufacturing”, SSRN, Link to preprint

Conference presentations:

Presented a poster at the 115th Annual General Meeting of the American Society for Microbiology in New Orleans, Louisiana, USA, 31 May 2015 to 02 June 2015

  1. Wenfa Ng (2015), “Colorless Agar for Enhancing Color Contrast between Colonies and Solid Medium”, Poster

Presented two posters at the Irish Division Meeting of the Society for General Microbiology at National University of Ireland, Galway, 17 to 19 June 2015

  1. Wenfa Ng (2015), “Possible odour-mediated attraction of flies to Bacillus subtilis NRS-762 stationary phase culture”, Poster
  2. Wenfa Ng (2015), “Conceptual basis and caveats in applying pattern recognition to mass spectrometry-based microbial identification”, Poster

Presented two papers at the 17th Regional Symposium on Chemical Engineering in Bangkok, Thailand, 22 to 23 November 2010

  1. Oral Presentation: Wenfa Ng and Yen-Peng Ting (2010), Formulation of a semi-defined medium for high cell density cultivation of Escherichia coli in shake flasks, Link to extended abstract
  2. Poster Presentation: Wenfa Ng and Yen-Peng Ting (2010), Zeta potential of bacterial cells: Effect of wash buffers, Link to extended abstract, Link to poster

Presented an oral presentation at the Workshop for Zhejiang University postgraduate students at the National University of Singapore, 24-25 July 2019

  1. Oral Presentation: Wenfa Ng (2019), “Exploring ethanol utilization for NADH regeneration in whole cell biocatalysis”, Link to presentation slides