Structural impediments to synthesis of bacterial cell wall

Article in Nature, Vol. 533, Issue 7604, pp. 557-560, “Structural insights into inhibition of lipid I production in bacterial cell wall synthesis”   Summary of article: Using X-ray diffraction, isothermal titration calorimetry and modelling, modes of binding and conformational rearrangement induced by binding of a synthesized inhibitor into a glycosyl transferase class enzyme was studied, … More Structural impediments to synthesis of bacterial cell wall

Architecture of multienzyme complex in action: polyketide synthase

Article in Nature, Vol. 531, 24 March 2016, pp. 533, “Mycocerosic acid synthase exemplifies the architecture of reducing polyketide synthases”   Summary of article: Various domains of polyketide synthases were crystallized individually or as segments, and the overall structure of the enzyme complex elucidated. While the method used structures of individual subunits to reconstruct the … More Architecture of multienzyme complex in action: polyketide synthase

The arduous process of drug discovery and development

Drug development is a continuous process. Specifically, there will always be drugs in preclinical development and more advanced ones in clinical trials. These medicines are typically exorbitant in cost due to a variety of reasons, the most important of which is their small production volume coupled with the need to recoup the hefty investment needed … More The arduous process of drug discovery and development

Elucidating molecular processes of molecules in dilute solutions with X-ray free electron laser spectroscopy

With diffraction quality crystals a rarity and some compounds and proteins almost impossible to crystallize, or in a crystal form without ligand in binding cavity, structural biology is often thought of as a field for the most patient. Why? Notwithstanding whether a particular target protein would form crystals of quality sufficient for structural elucidation, some … More Elucidating molecular processes of molecules in dilute solutions with X-ray free electron laser spectroscopy

Combining different experiment and instrument approaches to reconstruct how a protein moves and function

Structural biology is a field in biology research that seeks to understand the role of structure in potentiating function of an enzyme, receptor, or intracellular effector. To do that, it needs to first obtain the structure of the biomolecule of interest through either the gold standard X-ray diffraction technique or the more recent method of … More Combining different experiment and instrument approaches to reconstruct how a protein moves and function