Bacterial species in the Vibrio genus have two chromosomes instead of one

Genome sequencing studies have revealed that bacterial species in the Vibrio genus have two chromosomes instead of one. Typically, bacterial species genome is defined by one single circular chromosome, not two. The observations opened up many avenues for further research such as understanding the reasons why marine and aquatic Vibrio species are endowed with two … More Bacterial species in the Vibrio genus have two chromosomes instead of one

Generation of disease models through CRISPR-Cas genome editing technology

Lack of suitable disease models able to accurately recapitulate disease phenotypes is one of the major challenges in drug discovery and development given that disease models are needed for testing the relative efficacy of different candidate molecule at early stages of drug development. Thus, much effort have been devoted to the creation of suitable in … More Generation of disease models through CRISPR-Cas genome editing technology

Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Bacillus pumilus is a Gram-positive endospore forming bacterium capable of withstanding high intensity ultraviolet radiation. However, genome sequencing studies have revealed that the bacterium lack important genes in DNA repair compared to other bacterial species. Thus, it raises an important question concerning the mechanisms that underpins the enhanced resistance of the bacterium to high intensity … More Enhanced resistance to high intensity ultraviolet radiation in Bacillus pumilus SAFR-032

Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Glucose supplementation is commonly used to provide more carbon source to a medium to help facilitate the attainment of a greater cell density. However, from my personal experience in microbial cell culture, excessive glucose supplementation would reduce biomass formation, possibly due to the induction of overflow metabolism through the central carbon pathway that lead to … More Buffer system is necessary for a medium to moderate large pH fluctuation that arise from the metabolism of excess glucose

Different proteins could annotate a single mass peak of a mass spectrum

Functional annotation of mass spectrum of bacterial cells obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) generates the biological meaning that underpins the use of mass spectrometry for microbial identification. However, different proteins could have the same mass/charge ratio and thus able to annotate the same mass peak in a mass spectrum. Such … More Different proteins could annotate a single mass peak of a mass spectrum

Conserved mass peaks of bacteria at the genus level

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been validated to be useful in identifying many microbial species to the species level. However, the approach relies on the existence of different mass peaks between different microbial species. Such differences manifest as differences in mass peak distribution in mass spectra of microbes that could be … More Conserved mass peaks of bacteria at the genus level

Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Swarming motility is the collective coordinated movement of bacterial cells across a solid surface for colonizing new habitats such as during times of nutritional stress. Propelled by multiple flagella attached to the cell and aided by secreted surfactants and lubricants, swarming motility typically forms complex motility patterns during culture of the bacteria on solid agar … More Retarded swarming motility in Bacillus subtilis NRS-762 and Pseudomonas aeruginosa PRD-10

Informatic challenge in MALDI-TOF imaging mass spectrometry

Imaging mass spectrometry is a relatively new concept in mass spectrometry application that seeks to profile the ensemble of biomolecules in a tissue or sample. Useful for detecting and analysing biomolecules present in a sample in a large area format, imaging mass spectrometry generates huge datasets that must be annotated to obtain rich biological information … More Informatic challenge in MALDI-TOF imaging mass spectrometry

Theoretical ribosomal protein mass fingerprint database

Ribosomal proteins constitute the large and small subunit of the ribosome. Given the highly conserved nature of ribosomes, ribosomal proteins are expected to be similarly conserved. However, theoretical calculations of the molecular mass of ribosomal proteins of different species revealed the existence of distinct molecular mass fingerprint of ribosomal proteins from individual species. Such ribosomal … More Theoretical ribosomal protein mass fingerprint database

Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies

Bacillus subtilis is a common Gram-positive soil bacterium with important applications in biotechnology. Specifically, it is frequently used as the production host for a variety of value-added products or pharmaceuticals. Possessing a variety of differentiation pathways for coping with nutrient starvation, the bacterium is also a model organism for studying how bacteria cope with low … More Bacillus subtilis NRS-762 is not suitable as model organism for microbial survivability studies