Category: proteomics

SDS-PAGE uses molecular weight differences to separate different proteins

April 2, 2024

Gel electrophoresis is a common technique for separating proteins of different molecular weight. But, how does it work? Basically, the method known as sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) uses the effect of a constant applied electric field to induce movement of negatively charged proteins across a polyacrylamide gel. Specifically, the proteins are first … More SDS-PAGE uses molecular weight differences to separate different proteins

Suites of computational and experimental tools are in-place in modern biology to take advantage of high throughput experimental approaches

May 7, 2021

Modern biology is partially defined by the advent of omics approaches. These approaches could be genomics, proteomics, metabolomics, connectome or transcriptomics. But, they share one thing in common: large datasets are generated and provide guidance for downstream hypothesis driven methodologies. Generation of large datasets would mean that experimental approaches must correspondingly scale in size to … More Suites of computational and experimental tools are in-place in modern biology to take advantage of high throughput experimental approaches

Difficulty in defining a reference human proteome

May 6, 2020

Compared to genomics, proteomics is less data rich and ill-defined. This largely stems from the phenomenon of differential gene expression and protein production arising from different growth phases or nutritional and environmental conditions. Hence, it is theoretically difficult to define a reference human proteome, since a given proteome is only associated with a particular set … More Difficulty in defining a reference human proteome

Challenges of protein identification by MALDI-TOF mass spectrometry

December 9, 2017

The gold standard approach for identifying a protein is Western blot, which uses a primary antibody to recognize the target protein for subsequent visualization of the protein band by chemiluminescence. More recently, various mass spectrometry-based methods have been developed for the detection and identification of proteins.   These techniques could be classified into fragmentation-based methods … More Challenges of protein identification by MALDI-TOF mass spectrometry