Challenges of protein identification by MALDI-TOF mass spectrometry

The gold standard approach for identifying a protein is Western blot, which uses a primary antibody to recognize the target protein for subsequent visualization of the protein band by chemiluminescence. More recently, various mass spectrometry-based methods have been developed for the detection and identification of proteins.   These techniques could be classified into fragmentation-based methods … More Challenges of protein identification by MALDI-TOF mass spectrometry