Tag: growth medium

Yeast extract is a rich source of vitamins and growth factors for microbial culture

April 1, 2024

In the formulation of many microbial culture medium, it is inevitable to find yeast extract as a significant component of the complex medium. In simple terms, yeast extract is a rich source of vitamins and growth factors that enhance the microbiological medium, enabling it to culture more types of microbes, as well as take the … More Yeast extract is a rich source of vitamins and growth factors for microbial culture

Choice of growth medium depends on the mammalian cell type and stage of cancer

September 15, 2023

In general, students in the life sciences and biomedical research would think the DMEM medium is appropriate for most cell types. Indeed, that is true for most general applications. However, if you are asking more nuanced questions that require recapitulation of certain cellular phenotype or physiology, then modifications to DMEM or using some exquisite growth … More Choice of growth medium depends on the mammalian cell type and stage of cancer

Neuronal cells need high oxygen content and glucose for sustainment

June 11, 2023

Neuronal cell culture at the level of primary cells or cell lines is essential for creating an experimental model for studying a variety of neurological diseases such as dementia, Parkinson’s disease, different types of brain cancer. Typically, these neuronal cells need high oxygen content and glucose for optimal metabolic activity and cell sustainment and proliferation. … More Neuronal cells need high oxygen content and glucose for sustainment

Zeta potential of Escherichia coli DH5α cells grown in different growth media

January 15, 2020

Bacterial cells adapt to growth in different growth medium. Thus, could the cell surface structures and, by extension, surface charge characteristics of bacterial cells change as a result of growth in different growth medium. In addition, different growth medium comprises different sets of ions and metabolites for aiding growth of cells, could these ions and … More Zeta potential of Escherichia coli DH5α cells grown in different growth media

What is the amount of glucose that should be added to microbial growth medium?

January 9, 2020

Amount of glucose that should be present in a medium to ensure optimal biomass formation of the microorganism depends critically on the metabolism of the cells in relation to the production of acidic metabolites from glucose catabolism.   Glucose is metabolized by the central carbon pathway for the generation of ATP. If the amount of … More What is the amount of glucose that should be added to microbial growth medium?

Logic of growth for microbes

January 6, 2020

Energy obtained from nutrients are typically divided between cell maintenance and biomass formation. However, the proportion of nutritional energy that goes into either cell maintenance and biomass formation differs between different microbial species. Thus, what are the factors that govern the decision for partitioning energy between cell maintenance and biomass formation? And, does the amount … More Logic of growth for microbes

High cell density cultivation of Escherichia coli DH5α in shake flask

March 5, 2018

High cell density is desired in many biotechnology applications such as the generation of cells for characterization studies and performance tests in different scenarios. However, cell densities obtained during growth of bacteria in common growth media remained low; thereby, raising the issue of batch-to-batch variation in cell characteristics due to the need of using many … More High cell density cultivation of Escherichia coli DH5α in shake flask

Inability to constrain metabolic models with growth data in the absence of compositional changes in medium

December 25, 2017

The goal of metabolic modelling is to determine the metabolic flux through specific pathways in the cell. Doing so requires data on cell growth (whether microbes or human cells) as well as compositional data of the growth medium given that cellular metabolic activities would consume nutrients as well as generate byproducts that accumulate in the … More Inability to constrain metabolic models with growth data in the absence of compositional changes in medium