Transfer of laser energy from matrix coating helps prevent fragmentation of molecules

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) ionizes molecules without inducing fragmentation, thereby allowing molecular ion to be detected, which helps data interpretation of mass spectrum. The mechanisms underlying the ability of the mass spectrometry technique to ionize molecules without inducing fragmentation lies in the use of special matrix compounds that help transfer laser … More Transfer of laser energy from matrix coating helps prevent fragmentation of molecules

Is there a theoretical mass limit to MALDI-TOF mass spectrometry?

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) uses a laser to ionize molecules from a matrix coated sample surface, accelerate them along a vacuum tube through the application of an electric field, and obtained a mass/charge ratio of the molecular ion by timing the flight of the ion. Given that time-of-flight of the molecular … More Is there a theoretical mass limit to MALDI-TOF mass spectrometry?

How do we obtain structural information of protein?

Readers would often find scientific papers describing the structure of a single protein or molecule. However, given that we do not have the capability of manipulating single protein or molecule with ease, how do we image a single protein or molecule to obtain its structural information?   The answer lies in the use of X-ray … More How do we obtain structural information of protein?

Genomics enabled mass spectrometry-based proteomics

Mass spectrometry has developed into a critical tool for proteomics where a large number of studies had used mass spectrometry instruments for profiling the proteome of cells and tissues. However, identification of the mass peaks profiled in the mass spectrum would require the support of a library of molecular mass of all proteins profiled in … More Genomics enabled mass spectrometry-based proteomics

Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) generates microorganism-specific mass spectrum which has been shown to be useful in microbial identification via the mass spectrum fingerprinting approach. Specifically, the mass spectrum of an unknown microorganism is compared against those catalogued in a reference database of known microbes to aid in positive identification of microbe. … More Need for proteome molecular mass library to aid peak identification in MALDI-TOF MS microbial identification

Methods for powering an electric vehicle

Currently, direct charging of an electric vehicle is the only commercially available method for charging the vehicle. But, possibility exists in the use of replaceable power packs from existing network of petrol stations that provide the addon service of providing battery power packs for electric vehicles. Comparing the two different modes of providing electric charge … More Methods for powering an electric vehicle

Funding model for network of electric charging stations in a city

City-wide network of electric charging stations is necessary for supporting the use of electric cars in a city. However, current debate in the implementation of supporting infrastructure for electric cars in a city rest on the funding model for creation of such a city-wide electric charging network. Specifically, should the utilities companies in a city … More Funding model for network of electric charging stations in a city

Inability to constrain metabolic models with growth data in the absence of compositional changes in medium

The goal of metabolic modelling is to determine the metabolic flux through specific pathways in the cell. Doing so requires data on cell growth (whether microbes or human cells) as well as compositional data of the growth medium given that cellular metabolic activities would consume nutrients as well as generate byproducts that accumulate in the … More Inability to constrain metabolic models with growth data in the absence of compositional changes in medium

Phylogenetic trees track the evolutionary relatedness of genes and proteins

The tree of life is currently defined by a phylogenetic analysis of the 16S rRNA gene, which harbours deep evolutionary information on the development of various species on Earth. Specifically, it is highly conserved across species, and yet possesses sufficient variability for individual species to be identified by differential sequence at the gene level. Thus, … More Phylogenetic trees track the evolutionary relatedness of genes and proteins

Protein identification is the key challenge in proteomics

Proteomics seek to uncover the ensemble of proteins within a cell or the cell surface. However, whether assisted by mass spectrometry or Western Blot, the key challenge in proteomics remain the identification of proteins. Protein identification remains laborious and time expensive even in the case of mass spectrometry-based proteomics where the challenge lies in the … More Protein identification is the key challenge in proteomics