High precision sequencing of 16S rRNA gene, important for phylogenetic purposes

Article in PeerJ, “A method for high precision sequencing of near full-length 16S rRNA genes on an Illumina MiSeq”


Summary of article: A tag sequencing method utilizing the Ilumina MiSeq platform was shown to be useful in sequencing, in high precision, near full length 16S rRNA gene, which is important for phylogenetic purposes, particularly for microbial genotyping. With the new high speed and high precision sequencing machine, disease diagnostic in the event of an epidemic can be expedited for controlling disease spread by understanding the aetiological agent quicker and with better precision. In the research lab, the MiSeq enables large scale metagenomic screening of microbial 16S rRNA genes for understanding microbial community structure and relative abundances, two parameters important for determining ecosystem dynamics and health.


Link to original article: https://peerj.com/articles/2492/


Category: Interesting scientific articles, biochemistry, molecular biology, microbiology, microbiome,

Tags: phylogenetics, 16S rRNA gene, disease diagnostics, microbial metagenomics, microbial community structure,


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